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PMID: 1602560 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo genetic variability of the human immunodeficiency virus type 2 V3 region.

Journal of virology ·Vol. 66 ·No. 7 ·1992-07-00 ·Pages 4546-50

Boeri E, Giri A, Lillo F, Ferrari G, Varnier OE, Ferro A, Sabbatani S, Saxinger WC, Franchini G

Abstract

The principal neutralizing epitope of the human immunodeficiency virus type 1 (HIV-1) lies between two invariant cysteines in the third variable region (V3) of the viral envelope (gp120), and its amino acid sequence varies among different HIV-1 isolates. HIV-2 carries an analogous amino acid sequence between two cysteines of the V3 regions, but its functional similarity with the HIV-1 principal neutralizing epitope is uncertain. We studied the degree of genetic variation of the HIV-2 V3 region in fresh blood samples from 12 HIV-2-seropositive individuals from Guinea-Bissau. Polymerase chain reaction was used to amplify viral fragments of 465 bp containing the V3 region from cellular DNA. Nucleotide sequence analysis of the entire envelope fragment from each patient revealed that the degree of variation among field isolates of HIV-2 is comparable to that observed in the analogous region of HIV-1. Most of the HIV-2 isolates studied were highly related, suggesting the existence of a limited number of different viral strains in the cohort studied. Thus, the HIV-2 and HIV-1 V3 regions vary to a similar degree and may also have analogous functions.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Viral Gene Products, env/genetics Genetic Variation HIV Envelope Protein gp120/genetics HIV Seropositivity/microbiology HIV-1/genetics HIV-2/genetics Humans Molecular Sequence Data Peptide Fragments/genetics Polymerase Chain Reaction Protein Precursors/genetics Sequence Alignment Simian Immunodeficiency Virus/genetics Viral Envelope Proteins env Gene Products, Human Immunodeficiency Virus
Chemicals
DNA, Viral Gene Products, env HIV Envelope Protein gp120 HIV envelope protein gp120 (305-321) Peptide Fragments Protein Precursors Viral Envelope Proteins env Gene Products, Human Immunodeficiency Virus gp125 envelope protein, Human immunodeficiency virus 2
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Boeri E
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20892.
Giri A
Lillo F
Ferrari G
Varnier O E
Ferro A
Sabbatani S
Saxinger W C
Franchini G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.7.4546-4550.1992
Published
1992-07-00
Pages
4546-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241266
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.7.4546-4550.1992 PMC Full Text View journal details
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