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PMID: 1600942 Published · ppublish English Comparative Study Journal Article

Restin: a novel intermediate filament-associated protein highly expressed in the Reed-Sternberg cells of Hodgkin's disease.

The EMBO journal ·Vol. 11 ·No. 6 ·1992-06-00 ·Pages 2103-13

Bilbe G, Delabie J, Brüggen J, Richener H, Asselbergs FA, Cerletti N, Sorg C, Odink K, Tarcsay L, Wiesendanger W

Abstract

We have identified a cDNA coding for a protein of 160 kDa which is expressed in in vitro cultured human peripheral blood monocytes. The predicted amino acid sequence contains an alpha-helical rod domain possessing features characteristic of intermediate filament proteins. However, the immunocytochemical staining pattern, abundance and solubility in Triton X-100/high salt buffers suggest that this protein is probably only associated with the intermediate filament network and represents a new type of intermediate filament associated protein. In a survey of normal, inflammatory and human tumour tissue samples, this protein, which we have named restin, was found to be highly expressed in Reed-Sternberg cells, the tumoral cells diagnostic for Hodgkin's disease. We suggest that restin overexpression may be a contributing factor in the progression of Hodgkin's disease.

MeSH Terms
Amino Acid Sequence Animals Base Sequence CHO Cells Cell Line Cells, Cultured Cloning, Molecular Cricetinae DNA, Neoplasm/genetics,isolation & purification Gene Library Hodgkin Disease/genetics,metabolism Humans Intermediate Filament Proteins/genetics,isolation & purification,metabolism Microtubule-Associated Proteins Molecular Sequence Data Monocytes/cytology,metabolism,pathology Neoplasm Proteins/genetics,isolation & purification,metabolism Protein Conformation Reed-Sternberg Cells/metabolism Sequence Homology, Nucleic Acid Transfection Vimentin/analysis
Chemicals
DNA, Neoplasm Intermediate Filament Proteins Microtubule-Associated Proteins Neoplasm Proteins Vimentin cytoplasmic linker protein 170
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Bilbe G
Biotechnology, Pharma Division, Ciba-Geigy, Basel, Switzerland.
Delabie J
Brüggen J
Richener H
Asselbergs F A
Cerletti N
Sorg C
Odink K
Tarcsay L
Wiesendanger W
References (75)
75 references, click to expand
  1. Intermediate-sized filaments of human endothelial cells.
    J Cell Biol. 1979 Jun;81(3):570-80 PMID: 379021
  2. Different intermediate-sized filaments distinguished by immunofluorescence microscopy.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034-8 PMID: 368806
  3. Antibody to prekeratin. Decoration of tonofilament like arrays in various cells of epithelial character.
    Exp Cell Res. 1978 Oct 15;116(2):429-45 PMID: 361424
  4. 10 nm filaments in normal and transformed cells.
    Cell. 1978 Jan;13(1):151-63 PMID: 340048
  5. Coexistence of desmin and the fibroblastic intermediate filament subunit in muscle and nonmuscle cells: identification and comparative peptide analysis.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3894-8 PMID: 291047
  6. Isolation and characterization of glial filaments from human brain.
    J Cell Biol. 1978 Aug;78(2):426-40 PMID: 690174
  7. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  8. Structure of the gene encoding peripherin, an NGF-regulated neuronal-specific type III intermediate filament protein.
    Neuron. 1989 Jan;2(1):1043-53 PMID: 2624740
  9. Vimentin cDNA clones covering the complete intermediate-filament protein are found in an EHS tumor cDNA library.
    Gene. 1989 Mar 15;76(1):171-5 PMID: 2744479
  10. Antibodies to intermediate filament proteins in the diagnosis and classification of human tumors.
    Ultrastruct Pathol. 1984;7(2-3):83-107 PMID: 6085434
  11. Differential control of cytokeratins and vimentin synthesis by cell-cell contact and cell spreading in cultured epithelial cells.
    J Cell Biol. 1984 Oct;99(4 Pt 1):1424-33 PMID: 6207182
  12. Cloning and sequencing of rat plectin indicates a 466-kD polypeptide chain with a three-domain structure based on a central alpha-helical coiled coil.
    J Cell Biol. 1991 Jul;114(1):83-99 PMID: 2050743
  13. Protein kinase recognition sequence motifs.
    Trends Biochem Sci. 1990 Sep;15(9):342-6 PMID: 2238044
  14. Identification and characterization of a novel mammalian intermediate filament-associated protein.
    Cell Motil Cytoskeleton. 1990;17(1):19-33 PMID: 2225087
  15. Intermediate filaments.
    Curr Opin Cell Biol. 1989 Feb;1(1):36-43 PMID: 2698208
  16. Lamin B constitutes an intermediate filament attachment site at the nuclear envelope.
    J Cell Biol. 1987 Jul;105(1):117-25 PMID: 3301863
  17. Simian virus 40 enhancer increases RNA polymerase density within the linked gene.
    Nature. 1985 May 2-8;315(6014):75-7 PMID: 2986014
  18. The histogenesis of the Reed-Sternberg cell and its mononuclear counterparts.
    J Pathol. 1987 Mar;151(3):191-5 PMID: 3553510
  19. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  20. A recombinant Chinese hamster ovary cell line containing a 300-fold amplified tetramer of the hepatitis B genome together with a double selection marker expresses high levels of viral protein.
    J Mol Biol. 1986 Jun 5;189(3):401-11 PMID: 3023626
  21. Protein kinase activity associated with the nuclear lamina.
    Proc Natl Acad Sci U S A. 1988 May;85(9):2994-8 PMID: 2834726
  22. A long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mRNA in murine cytomegalovirus.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8325-9 PMID: 3001696
  23. Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.
    Eur J Biochem. 1987 Jul 1;166(1):71-7 PMID: 3036526
  24. Cytoskeletal intermediate filaments: practical applications of intermediate filament analysis.
    Ultrastruct Pathol. 1987;11(2-3):175-89 PMID: 3035766
  25. Site specificity in vimentin-membrane interactions: intermediate filament subunits associate with the plasma membrane via their head domains.
    J Cell Biol. 1985 Jun;100(6):1962-7 PMID: 3158665
  26. The binding of vimentin to human erythrocyte membranes: a model system for the study of intermediate filament-membrane interactions.
    J Cell Biol. 1985 Jun;100(6):1955-61 PMID: 3158664
  27. Molecular and cellular biology of intermediate filaments.
    Annu Rev Biochem. 1988;57:593-625 PMID: 3052284
  28. A second higher vertebrate B-type lamin. cDNA sequence determination and in vitro processing of chicken lamin B2.
    J Mol Biol. 1989 Aug 5;208(3):405-15 PMID: 2477550
  29. Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments.
    J Cell Biol. 1989 Apr;108(4):1477-93 PMID: 2466849
  30. Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein.
    J Neurosci. 1987 Aug;7(8):2590-9 PMID: 2441012
  31. Induction of cytokeratin expression in human mesenchymal cells.
    J Cell Physiol. 1987 Nov;133(2):321-9 PMID: 2445764
  32. Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation.
    Differentiation. 1986;33(1):61-8 PMID: 2434380
  33. Characterization of two monoclonal antibodies to human epidermal keratohyalin: reactivity with filaggrin and related proteins.
    J Invest Dermatol. 1987 Mar;88(3):306-13 PMID: 2434577
  34. Mesenchymal-epithelial conversions induced by 5-azacytidine: appearance of cytokeratin Endo-A messenger RNA.
    Differentiation. 1986;31(1):61-6 PMID: 2426152
  35. Amino acid sequences of mouse and human epidermal type II keratins of Mr 67,000 provide a systematic basis for the structural and functional diversity of the end domains of keratin intermediate filament subunits.
    J Biol Chem. 1985 Jun 10;260(11):7142-9 PMID: 2581964
  36. Coexpression of intermediate filaments in normal and neoplastic human tissues: a reappraisal.
    Ultrastruct Pathol. 1989 Sep-Dec;13(5-6):501-14 PMID: 2678645
  37. Phosphorylation and disassembly of intermediate filaments in mitotic cells.
    Proc Natl Acad Sci U S A. 1989 Mar;86(6):1885-9 PMID: 2648386
  38. Differentiation and retrodifferentiation of U937 cells: reversible induction and suppression of intermediate filament protein synthesis.
    Eur J Cell Biol. 1990 Apr;51(2):265-71 PMID: 2351153
  39. The predicted amino acid sequence of alpha-internexin is that of a novel neuronal intermediate filament protein.
    EMBO J. 1990 Mar;9(3):749-55 PMID: 2311576
  40. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.
    Cell. 1981 Dec;27(2 Pt 1):279-88 PMID: 6277501
  41. Immunochemical identification of intermediate-sized filaments in human neoplastic cells. A diagnostic aid for the surgical pathologist.
    Am J Pathol. 1981 Sep;104(3):206-16 PMID: 6170230
  42. Use of antibodies to intermediate filaments in the characterization of human tumors.
    Cold Spring Harb Symp Quant Biol. 1982;46 Pt 1:331-9 PMID: 6179695
  43. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.
    Lab Invest. 1983 Apr;48(4):372-94 PMID: 6187996
  44. Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis.
    Nature. 1987 Nov 5-11;330(6143):80-2 PMID: 3313057
  45. Generation and characterization of a monoclonal antibody (1C5) to human migration inhibitory factor (MIF).
    Immunobiology. 1986 Jul;171(4-5):461-74 PMID: 3527953
  46. Protein and amino acid composition of hair from mice carrying the naked (N) gene.
    Genet Res. 1984 Aug;44(1):29-38 PMID: 6489753
  47. Coding sequence and growth regulation of the human vimentin gene.
    Mol Cell Biol. 1986 Nov;6(11):3614-20 PMID: 3467175
  48. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
    Nature. 1986 Feb 6-12;319(6053):463-8 PMID: 3453101
  49. The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family.
    EMBO J. 1987 Jun;6(6):1617-26 PMID: 3608989
  50. Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A.
    Nucleic Acids Res. 1985 Oct 11;13(19):6867-80 PMID: 3840589
  51. Anti-vimentin antibody reactivity with Reed-Sternberg cells of Hodgkin's disease.
    Virchows Arch A Pathol Anat Histopathol. 1990;417(1):43-8 PMID: 1694331
  52. Disruption of cytoskeletal structures results in the induction of the urokinase-type plasminogen activator gene expression.
    J Biol Chem. 1990 Aug 5;265(22):13327-34 PMID: 1695907
  53. CNS stem cells express a new class of intermediate filament protein.
    Cell. 1990 Feb 23;60(4):585-95 PMID: 1689217
  54. Intermediate filament dynamics.
    Cell. 1990 Feb 23;60(4):521-3 PMID: 2406023
  55. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  56. Intermediate filament-associated proteins.
    Curr Opin Cell Biol. 1991 Feb;3(1):75-81 PMID: 1854487
  57. Colon cancer. Consider the coiled coil....
    Nature. 1991 May 16;351(6323):188-90 PMID: 1645848
  58. Identification of deletion mutations and three new genes at the familial polyposis locus.
    Cell. 1991 Aug 9;66(3):601-13 PMID: 1678319
  59. Colon cancer. Suppression with a difference.
    Nature. 1991 Oct 24;353(6346):696-7 PMID: 1658657
  60. Mutations of chromosome 5q21 genes in FAP and colorectal cancer patients.
    Science. 1991 Aug 9;253(5020):665-9 PMID: 1651563
  61. Identification of FAP locus genes from chromosome 5q21.
    Science. 1991 Aug 9;253(5020):661-5 PMID: 1651562
  62. Identification and characterization of the familial adenomatous polyposis coli gene.
    Cell. 1991 Aug 9;66(3):589-600 PMID: 1651174
  63. Epithelial layer formation in differentiating aggregates of F9 embryonal carcinoma cells.
    J Cell Biol. 1983 Jun;96(6):1690-6 PMID: 6343402
  64. Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2743-6 PMID: 6585825
  65. Detection of phenotypic differences on human malignant melanoma lines and their variant sublines with monoclonal antibodies.
    Cancer Immunol Immunother. 1983;15(3):200-5 PMID: 6555061
  66. Heterogeneity of primary and metastatic human malignant melanoma as detected with monoclonal antibodies in cryostat sections of biopsies.
    Cancer Immunol Immunother. 1983;16(1):53-8 PMID: 6556951
  67. Amino acid sequence data on glial fibrillary acidic protein (GFA); implications for the subdivision of intermediate filaments into epithelial and non-epithelial members.
    EMBO J. 1983;2(11):2059-63 PMID: 6685624
  68. Reorganization of cytoskeletal and contractile elements during transition of human monocytes into adherent macrophages.
    Lab Invest. 1982 Oct;47(4):391-9 PMID: 6811799
  69. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4216-20 PMID: 6933469
  70. The synthetic chemotactic peptide formyl-methionyl-leucyl-phenylalanine causes an increase in actin associated with the cytoskeleton in rabbit neutrophils.
    Biochem Biophys Res Commun. 1982 Oct 15;108(3):1144-9 PMID: 6891213
  71. Intermediate filaments as mechanical integrators of cellular space.
    Nature. 1980 Jan 17;283(5744):249-256 PMID: 7188712
  72. Intermediate filaments from Chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells.
    J Biol Chem. 1981 Feb 10;256(3):1428-31 PMID: 7451510
  73. Intermediate filaments in nervous tissues.
    J Cell Biol. 1978 Dec;79(3):637-45 PMID: 83322
  74. Isolation of histone kinases.
    Methods Cell Biol. 1978;19:143-52 PMID: 211394
  75. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-06-00
Pages
2103-13
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556677
Subset
IM
Databases
GENBANK
X64335, X64555, X64556, X64629, X64700, X64838, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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