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PMID: 15998808 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A systematic RNAi screen for longevity genes in C. elegans.

Genes & development ·Vol. 19 ·No. 13 ·2005-07-01 ·Pages 1544-55

Hamilton B, Dong Y, Shindo M, Liu W, Odell I, Ruvkun G, Lee SS

Abstract

We report here the first genome-wide functional genomic screen for longevity genes. We systematically surveyed Caenorhabditis elegans genes using large-scale RNA interference (RNAi), and found that RNAi inactivation of 89 genes extend C. elegans lifespan. Components of the daf-2/insulin-like signaling pathway are recovered, as well as genes that regulate metabolism, signal transduction, protein turnover, and gene expression. Many of these candidate longevity genes are conserved across animal phylogeny. Genetic interaction analyses with the new longevity genes indicate that some act upstream of the daf-16/FOXO transcription factor or the sir2.1 protein deacetylase, and others function independently of daf-16/FOXO and sir2.1, and might define new pathways to regulate lifespan.

MeSH Terms
Animals Caenorhabditis elegans/genetics Longevity/genetics RNA Interference
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hamilton Benjamin
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14850, USA.
Dong Yuqing
Shindo Mami
Liu Wenyu
Odell Ian
Ruvkun Gary
Lee Siu Sylvia
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1308205
Published
2005-07-01
Pages
1544-55
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1172061
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1308205 PMC Full Text View journal details
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