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PMID: 15986205 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Developmental regulation of Suz 12 localization.

Chromosoma ·Vol. 114 ·No. 3 ·2005-08-00 ·Pages 183-92

de la Cruz CC, Fang J, Plath K, Worringer KA, Nusinow DA, Zhang Y, Panning B

Abstract

Chromatin modifications are among the epigenetic alterations essential for genetic reprogramming during development. The Polycomb group (PcG) gene family mediates chromatin modifications that contribute to developmentally regulated transcriptional silencing. Trimethylation of histone H3 on lysine 27, mediated by a PcG protein complex consisting of Eed, Ezh2, and Suz12, is integral in differentiation, stem cell self-renewal, and tumorigenesis. Eed and Ezh2 are also implicated in the developmentally regulated silencing of the inactive X chromosome, as they are transiently enriched on the inactive X chromosome when X chromosome silencing is initiated. Here we analyze the dynamic localization of Suz12 during cellular differentiation and X-inactivation. Though Suz12 is a requisite member of the Eed/Ezh2 complexes, we found that Suz12 exhibits a notable difference from Ezh2 and Eed: while Ezh2 and Eed levels decrease during stem cell differentiation, Suz12 levels remain constant. Despite the differential regulation in abundance of Suz12 and Eed/Ezh2, Suz12 is also transiently enriched on the Xi during early stages of X-inactivation, and this accumulation is Xist RNA dependent. These results suggest that Suz12 may have a function that is not mediated by its association with Eed and Ezh2, and that this additional function is not involved in the regulation of X-inactivation.

MeSH Terms
Animals Carrier Proteins/genetics,metabolism,physiology Cell Differentiation DNA-Binding Proteins/metabolism Gene Expression Regulation, Developmental/genetics,physiology Histone Methyltransferases Histone-Lysine N-Methyltransferase/metabolism Histones/metabolism Humans Methylation Mice Neoplasm Proteins Nuclear Proteins Polycomb Repressive Complex 2 Protein Methyltransferases RNA, Long Noncoding RNA, Small Interfering/genetics,metabolism RNA, Untranslated/metabolism Recombinant Fusion Proteins/metabolism Repressor Proteins/metabolism Stem Cells/metabolism Transcription Factors/metabolism Transfection Trophoblasts/cytology,metabolism X Chromosome Inactivation
Chemicals
Carrier Proteins DNA-Binding Proteins EED protein, human Histones Neoplasm Proteins Nuclear Proteins RNA, Long Noncoding RNA, Small Interfering RNA, Untranslated Recombinant Fusion Proteins Repressor Proteins SUZ12 protein, human Transcription Factors XIST non-coding RNA Histone Methyltransferases Protein Methyltransferases EZH1 protein, human Histone-Lysine N-Methyltransferase Polycomb Repressive Complex 2
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
de la Cruz Cecile C
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
Fang Jia
Plath Kathrin
Worringer Kathleen A
Nusinow Dmitri A
Zhang Yi
Panning Barbara
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/s00412-005-0008-6
Published
2005-08-00
Epub
2005-00-29
Pages
183-92
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · R01 GM063671 · United States
External Links
PubMed source DOI: 10.1007/s00412-005-0008-6 View journal details
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