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PMID: 1594628 Published · ppublish English Journal Article

Transforming growth factor beta induces myoblast differentiation in the presence of mitogens.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 11 ·1992-06-01 ·Pages 5176-80

Zentella A, Massagué J

Abstract

Transforming growth factor beta 1 (TGF-beta 1) added to L6E9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the G1 phase of the cell cycle. This growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. These results suggest that TGF-beta 1 may act as a physiological inducer of myogenic differentiation in mitogen-rich environments, and its otherwise reversible growth inhibitory effect may become permanent if coupled to induction of terminal differentiation.

MeSH Terms
Animals Base Sequence Cell Cycle/drug effects Cell Differentiation/drug effects Cells, Cultured Creatine Kinase/metabolism Gene Expression/drug effects Genes, myc In Vitro Techniques Mitogens/pharmacology Molecular Sequence Data Muscles/cytology RNA, Messenger/genetics Rats Transforming Growth Factor beta/pharmacology
Chemicals
Mitogens RNA, Messenger Transforming Growth Factor beta Creatine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zentella A
Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Massagué J
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51 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.11.5176
Published
1992-06-01
Pages
5176-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49252
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.11.5176 PMC Full Text View journal details
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