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PMID: 1594586 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 11 ·1992-06-01 ·Pages 4869-73

Morris KN, Wool IG

Abstract

The A chain of ricin is a RNA N-glycosidase that inactivates ribosomes by depurination of a single adenosine in 28S rRNA. Of the 267 amino acids in the protein, 222 (83%) could be deleted from one or another of 74 mutants without loss of the capacity of the protein to recognize a single nucleotide from among the 7000 in ribosomes or to catalyze hydrolysis.

MeSH Terms
Amino Acid Sequence Cloning, Molecular Computer Graphics DNA Mutational Analysis Models, Molecular Molecular Sequence Data Protein Conformation Ricin/chemistry,metabolism,toxicity Structure-Activity Relationship
Chemicals
Ricin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morris K N
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
Wool I G
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.11.4869
Published
1992-06-01
Pages
4869-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49189
Subset
IM
Grants
NIGMS NIH HHS · GM 33702 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.11.4869 PMC Full Text View journal details
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