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PMID: 15944366 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Neutral evolution of the nonbinding region of the anthocyanin regulatory gene Ipmyb1 in Ipomoea.

Genetics ·Vol. 170 ·No. 4 ·2005-08-00 ·Pages 1967-78

Chang SM, Lu Y, Rausher MD

Abstract

Plant transcription factors often contain domains that evolve very rapidly. Although it has been suggested that this rapid evolution may contribute substantially to phenotypic differentiation among species, this suggestion has seldom been tested explicitly. We tested the validity of this hypothesis by examining the rapidly evolving non-DNA-binding region of an R2R3-myb transcription factor that regulates anthocyanin expression in flowers of the genus Ipomoea. We first provide evidence that the W locus in Ipomoea purpurea, which determines whether flowers will be pigmented or white, corresponds to a myb gene segregating in southeastern U.S. populations for one functional allele and one nonfunctional allele. While the binding domain exhibits substantial selective constraint, the nonbinding region evolves at an average K(a)/K(s) ratio of 0.74. This elevated rate of evolution is due to relaxed constraint rather than to increased levels of positive selection. Despite this relaxed constraint, however, approximately 20-25% of the codons, randomly distributed throughout the nonbinding region, are highly constrained, with the remainder evolving neutrally, indicating that the entire region performs important function(s). Our results provide little indication that rapid evolution in this regulatory gene is driven by natural selection or that it is responsible for floral-color differences among Ipomoea species.

MeSH Terms
Alleles Amino Acid Sequence Amino Acid Substitution Anthocyanins/chemistry,genetics Cloning, Molecular Codon Computer Simulation Evolution, Molecular Genes, Plant Genes, Regulator Genetic Variation Ipomoea/genetics Kinetics Molecular Sequence Data Phylogeny Protein Structure, Tertiary Selection, Genetic Sequence Homology, Amino Acid
Chemicals
Anthocyanins Codon
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chang Shu-Mei
Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA.
Lu Yingqing
Rausher Mark D
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.034975
Published
2005-08-00
Epub
2005-00-08
Pages
1967-78
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449781
Subset
IM
Databases
GENBANK
AY986823, AY986824, AY986825, AY986826, AY986827, AY986828, AY986829, AY986830, AY986831, AY986832, AY986833, AY986834, AY986835, AY986836, AY986837, AY986838, AY986839, AY986840, AY986841, AY986842, AY986843, AY986844, AY986845, AY986846, AY986847, AY986848, AY986849, AY986850, AY986851, AY986852, AY986853, AY986854, AY986855, AY986856, AY986857, AY986858, AY986859, AY986860, AY986861, AY986862, AY986863, AY986864, AY986865, AY986866, AY986867, AY986868, AY986869, AY986870, AY986871, AY986872, AY986873, AY986874, AY986875, AY986876, AY986877, AY986878, AY986879, AY986880, AY986881, AY986882, AY986883, AY986884, AY986885, AY986886, AY986887, AY986888, AY986889, AY986890, AY986891, AY986892, AY986893, AY986894, AY986895, AY986896, AY986897, AY986898
External Links
PubMed source DOI: 10.1534/genetics.104.034975 PMC Full Text View journal details
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