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PMID: 1584756 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alternative usage of initiation codons in mRNA encoding the cAMP-responsive-element modulator generates regulators with opposite functions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 10 ·1992-05-15 ·Pages 4226-30

Delmas V, Laoide BM, Masquilier D, de Groot RP, Foulkes NS, Sassone-Corsi P

Abstract

The cAMP-responsive-element modulator (CREM) gene encodes both antagonists (CREM alpha/beta/gamma) and an activator (CREM tau) of cAMP-responsive transcription by alternative splicing. In adult mouse brain a predominant 21-kDa protein, not corresponding to any previously characterized transcript, is detected with specific CREM antibodies. A developmental switch occurs in brain as expression changes at birth from CREM alpha/beta to the 21-kDa protein. We show that the 21-kDa protein corresponds to S-CREM (short CREM), a protein produced by the use of an internal AUG initiation codon in the CREM tau transcript. S-CREM shares with the other CREM proteins the basic DNA-binding and leucine-zipper dimerization domain. S-CREM functions as a transcriptional repressor of cAMP-induced transcription. Thus, two proteins with opposite functions are generated by alternative translation using two AUG codons within the same reading frame.

MeSH Terms

Animals Brain/physiology Cell Line Chloramphenicol O-Acetyltransferase/genetics,metabolism Chromosome Deletion Codon Cyclic AMP Response Element Modulator DNA-Binding Proteins/genetics Genes, Regulator Genetic Vectors Mice RNA Splicing RNA, Messenger/genetics Repressor Proteins Transcription, Genetic Transfection

Chemicals

Codon DNA-Binding Proteins RNA, Messenger Repressor Proteins Cyclic AMP Response Element Modulator Chloramphenicol O-Acetyltransferase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Delmas V

Laboratoire De Génétique Moléculaire des Eucaryotes, U184 Institut National de la Santé et de la Recherche Médicale, Faculté de Medecine, Strasbourg, France.

Laoide B M

Masquilier D

de Groot R P

Foulkes N S

Sassone-Corsi P

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.89.10.4226

Published

1992-05-15

Pages

4226-30

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC49054

Subset

External Links

PubMed source DOI: 10.1073/pnas.89.10.4226 PMC Full Text View journal details

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Alternative usage of initiation codons in mRNA encoding the cAMP-responsive-element modulator generates regulators with opposite functions.

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