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PMID: 1825810 Published · ppublish English Comparative Study Journal Article

The pim-1 oncogene encodes two related protein-serine/threonine kinases by alternative initiation at AUG and CUG.

The EMBO journal ·Vol. 10 ·No. 3 ·1991-03-00 ·Pages 655-64

Saris CJ, Domen J, Berns A

Abstract

The pim-1 gene is frequently found activated by proviral insertion in murine T cell lymphomas. Overexpression of pim-1 in lymphoid cells by transgenesis formally proved its oncogenic potential. The pim-1 cDNA sequence predicts that both murine and human pim-1 encode a 34 kd protein with homology to protein kinases. In this study, we show that the murine pim-1 gene encodes a 44 kd protein in addition to the predicted 34 kd protein. The 44 kd protein is an amino-terminal extension of the 34 kd protein and is synthesized by alternative translation initiation at an upstream CUG codon. Contrary to previous findings by others, we provide evidence that both murine and human pim-1 gene products are protein-serine/threonine kinases. Murine 44 kd and 34 kd pim-1 proteins exhibit comparable in vitro kinase activity and are both mainly cytoplasmic, but they differ in in vivo association state and half-life.

Related Genes
pim-1
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Codon/genetics Humans Kinetics Mice Molecular Sequence Data Mutagenesis, Site-Directed Oligonucleotide Probes Peptide Mapping Protein Biosynthesis Protein Kinases/genetics Protein Serine-Threonine Kinases Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-pim-1 Proto-Oncogenes Sequence Homology, Nucleic Acid Transfection
Chemicals
Codon Oligonucleotide Probes Proto-Oncogene Proteins Protein Kinases PIM1 protein, human Pim1 protein, mouse Protein Serine-Threonine Kinases Proto-Oncogene Proteins c-pim-1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saris C J
Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam.
Domen J
Berns A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-03-00
Pages
655-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452698
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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