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PMID: 1582554 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Commitment to meiosis in Saccharomyces cerevisiae: involvement of the SPO14 gene.

Genetics ·Vol. 130 ·No. 4 ·1992-04-00 ·Pages 703-16

Honigberg SM, Conicella C, Espositio RE

Abstract

This paper describes the identification, cloning and phenotypic analysis of SPO14, a new gene required for meiosis and spore formation. Studies of strains carrying a temperature-sensitive mutation or a disruption/duplication allele indicate that spo14 mutants have the unusual property of being able to return to mitotic division, even from the late stages of meiotic development. Early meiotic events, such as DNA replication and intragenic and intergenic recombination, occur normally. In contrast, later meiotic processes are defective in spo14 mutants: the meiosis I division appears to be executed at slightly depressed levels, the meiosis II division is reduced more severely, and no spores are formed. Epistasis tests using mutants defective in recombination or reductional division support these findings. Based on these data, we suggest that the SPO14 gene product is involved in the coordinate induction of late meiotic events and that this induction is responsible for the phenomenon of commitment.

Related Genes
SPO14 spo14
MeSH Terms
Cloning, Molecular Genes, Fungal/genetics,physiology Meiosis/genetics Mutation Saccharomyces cerevisiae/genetics,growth & development Spores, Fungal/physiology
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Honigberg S M
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Conicella C
Espositio R E
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/130.4.703
Published
1992-04-00
Pages
703-16
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1204922
Subset
IM
Grants
NIGMS NIH HHS · GM298182 · United States
NICHD NIH HHS · HD19252 · United States
External Links
PubMed source DOI: 10.1093/genetics/130.4.703 PMC Full Text View journal details
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