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PMID: 15800061 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules.

Molecular biology of the cell ·Vol. 16 ·No. 6 ·2005-06-00 ·Pages 2786-98

Gatfield J, Albrecht I, Zanolari B, Steinmetz MO, Pieters J

Abstract

Coronin 1 is a member of the coronin protein family specifically expressed in leukocytes and accumulates at sites of rearrangements of the F-actin cytoskeleton. Here, we describe that coronin 1 molecules are coiled coil-mediated homotrimeric complexes, which associate with the plasma membrane and with the cytoskeleton via two distinct domains. Association with the cytoskeleton was mediated by trimerization of a stretch of positively charged residues within a linker region between the N-terminal, WD repeat-containing domain and the C-terminal coiled coil. In contrast, neither the coiled coil nor the positively charged residues within the linker domain were required for plasma membrane binding, suggesting that the N-terminal, WD repeat-containing domain mediates membrane interaction. The capacity of coronin 1 to link the leukocyte cytoskeleton to the plasma membrane may serve to integrate outside-inside signaling with modulation of the cytoskeleton.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Cell Line Cell Membrane/metabolism Circular Dichroism Cytoskeleton/metabolism Electrophoresis, Gel, Two-Dimensional Humans Immunohistochemistry Introns Jurkat Cells Leukocytes/cytology Macrophages/cytology Mice Microfilament Proteins/chemistry,genetics,isolation & purification,metabolism,ultrastructure Models, Biological Molecular Sequence Data Protein Isoforms/chemistry,genetics,metabolism Protein Structure, Secondary Protein Structure, Tertiary Sequence Analysis, Protein Sequence Homology, Amino Acid Spectrophotometry, Ultraviolet Subcellular Fractions/metabolism
Chemicals
Actins Microfilament Proteins Protein Isoforms coronin proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gatfield John
Biozentrum, University of Basel, CH 4056 Basel, Switzerland.
Albrecht Imke
Zanolari Bettina
Steinmetz Michel O
Pieters Jean
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-01-0042
Published
2005-06-00
Epub
2005-00-30
Pages
2786-98
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1142424
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e05-01-0042 PMC Full Text View journal details
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