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PMID: 15767671 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that the elongation factor TFIIS plays a role in transcription initiation at GAL1 in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 25 ·No. 7 ·2005-04-00 ·Pages 2650-9

Prather DM, Larschan E, Winston F

Abstract

TFIIS is a transcription elongation factor that has been extensively studied biochemically. Although the in vitro mechanisms by which TFIIS stimulates RNA transcript cleavage and polymerase read-through have been well characterized, its in vivo roles remain unclear. To better understand TFIIS function in vivo, we have examined its role during Gal4-mediated activation of the Saccharomyces cerevisiae GAL1 gene. Surprisingly, TFIIS is strongly associated with the GAL1 upstream activating sequence. In addition, TFIIS recruitment to Gal4-binding sites is dependent on Gal4, SAGA, and Mediator but not on RNA polymerase II (Pol II). The association of TFIIS is also necessary for the optimal recruitment of TATA-binding protein and Pol II to the GAL1 promoter. These results provide strong evidence that TFIIS plays an important role in the initiation of transcription at GAL1 in addition to its well-characterized roles in transcription elongation.

MeSH Terms
Binding Sites DNA-Binding Proteins Galactokinase/genetics Gene Expression Regulation, Fungal Genes, Fungal/genetics Promoter Regions, Genetic/genetics Protein Binding RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Temperature Transcription Factors/metabolism Transcription, Genetic/genetics Transcriptional Elongation Factors/genetics,metabolism
Chemicals
DNA-Binding Proteins GAL4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcriptional Elongation Factors transcription factor S-II Galactokinase RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Prather Donald M
Department of Genetics, Harvard Medical School, 77 Louis Pasteur Ave., Boston, MA 02115, USA.
Larschan Erica
Winston Fred
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.25.7.2650-2659.2005
Published
2005-04-00
Pages
2650-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1061654
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045720 · United States
NIGMS NIH HHS · GM45720 · United States
External Links
PubMed source DOI: 10.1128/MCB.25.7.2650-2659.2005 PMC Full Text View journal details
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