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PMID: 15743955 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli.

Journal of bacteriology ·Vol. 187 ·No. 6 ·2005-03-00 ·Pages 2066-76

Wang L, Hashimoto Y, Tsao CY, Valdes JJ, Bentley WE

Abstract

Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes.

MeSH Terms
Bacterial Proteins/genetics Carbon-Sulfur Lyases Cyclic AMP/metabolism Cyclic AMP Receptor Protein Escherichia coli/genetics,metabolism Escherichia coli Proteins/genetics,metabolism Fermentation/physiology Gene Expression Regulation, Bacterial Glucose/metabolism Homoserine/analogs & derivatives,biosynthesis,metabolism Lactones/metabolism Mutagenesis N-Glycosyl Hydrolases/genetics Operon/genetics Promoter Regions, Genetic/physiology Receptors, Cell Surface/genetics,metabolism Receptors, Cyclic AMP/metabolism Transcription Factors/genetics,metabolism
Chemicals
Bacterial Proteins Cyclic AMP Receptor Protein Escherichia coli Proteins Lactones N-octanoylhomoserine lactone Receptors, Cell Surface Receptors, Cyclic AMP Transcription Factors crp protein, E coli Homoserine Cyclic AMP N-Glycosyl Hydrolases adenosylhomocysteine nucleosidase Carbon-Sulfur Lyases LuxS protein, Bacteria Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang Liang
Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA.
Hashimoto Yoshifumi
Tsao Chen-Yu
Valdes James J
Bentley William E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.6.2066-2076.2005
Published
2005-03-00
Pages
2066-76
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1064054
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.187.6.2066-2076.2005 PMC Full Text View journal details
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