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PMID: 15728719 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A mutation in dVps28 reveals a link between a subunit of the endosomal sorting complex required for transport-I complex and the actin cytoskeleton in Drosophila.

Molecular biology of the cell ·Vol. 16 ·No. 5 ·2005-05-00 ·Pages 2301-12

Sevrioukov EA, Moghrabi N, Kuhn M, Krämer H

Abstract

Proteins that constitute the endosomal sorting complex required for transport (ESCRT) are necessary for the sorting of proteins into multivesicular bodies (MVBs) and the budding of several enveloped viruses, including HIV-1. The first of these complexes, ESCRT-I, consists of three proteins: Vps28p, Vps37p, and Vps23p or Tsg101 in mammals. Here, we characterize a mutation in the Drosophila homolog of vps28. The dVps28 gene is essential: homozygous mutants die at the transition from the first to second instar. Removal of maternally contributed dVps28 causes early embryonic lethality. In such embryos lacking dVps28, several processes that require the actin cytoskeleton are perturbed, including axial migration of nuclei, formation of transient furrows during cortical divisions in syncytial embryos, and the subsequent cellularization. Defects in actin cytoskeleton organization also become apparent during sperm individualization in dVps28 mutant testis. Because dVps28 mutant cells contained MVBs, these defects are unlikely to be a secondary consequence of disrupted MVB formation and suggest an interaction between the actin cytoskeleton and endosomal membranes in Drosophila embryos earlier than previously appreciated.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Cytoskeleton/metabolism Drosophila/embryology,genetics,metabolism Drosophila Proteins/genetics,metabolism Endosomes/metabolism Female Genes, Insect Male Microscopy, Electron Molecular Sequence Data Mutation Sequence Homology, Amino Acid Spermatogenesis Vesicular Transport Proteins/genetics,metabolism
Chemicals
Actins Drosophila Proteins Vesicular Transport Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sevrioukov Evgueni A
Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9111, USA.
Moghrabi Nabil
Kuhn Mary
Krämer Helmut
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-11-1013
Published
2005-05-00
Epub
2005-00-23
Pages
2301-12
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1087236
Subset
IM
Grants
NEI NIH HHS · R01 EY010199 · United States
NINDS NIH HHS · R01 NS043406 · United States
NEI NIH HHS · EY-10199 · United States
NINDS NIH HHS · NS-43406 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-11-1013 PMC Full Text View journal details
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