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PMID: 15711971 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana.

Molecular genetics and genomics : MGG ·Vol. 273 ·No. 1 ·2005-03-00 ·Pages 10-9

Berrin JG, Pierrugues O, Brutesco C, Alonso B, Montillet JL, Roby D, Kazmaier M

Abstract

A novel Arabidopsis thaliana gene (AtNADK-1) was identified based on its response to radiation and oxidative stress. Levels of AtNADK-1 mRNA increase eight-fold following exposure to ionising radiation and are enhanced three-fold by treatment with hydrogen peroxide. The gene also appears to be differentially regulated during compatible and incompatible plant-pathogen interactions in response to Pseudomonas syringae pv. tomato. The full-length AtNADK-1 cDNA encodes a 58-kDa protein that shows high sequence homology to the recently defined family of NAD(H) kinases. Recombinant AtNADK-1 utilises ATP to phosphorylate both NAD and NADH, showing a two-fold preference for NADH. Using reverse genetics, we demonstrate that AtNADK-1 deficient plants display enhanced sensitivity to gamma irradiation and to paraquat-induced oxidative stress. Our results indicate that this novel NAD(H) kinase may contribute to the maintenance of redox status in Arabidopsis thaliana.

MeSH Terms
Amino Acid Sequence Anthocyanins/metabolism Arabidopsis/genetics,metabolism,microbiology Arabidopsis Proteins/genetics,metabolism Base Sequence DNA Primers DNA Transposable Elements/genetics DNA, Complementary/genetics Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Plant/drug effects,radiation effects Hydrogen Peroxide/pharmacology Molecular Sequence Data Oxidative Stress/genetics Phosphotransferases/genetics,metabolism Phosphotransferases (Alcohol Group Acceptor)/genetics,metabolism Pseudomonas syringae Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA
Chemicals
Anthocyanins Arabidopsis Proteins DNA Primers DNA Transposable Elements DNA, Complementary Hydrogen Peroxide Phosphotransferases NADK-1 protein, Arabidopsis Phosphotransferases (Alcohol Group Acceptor)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Berrin Jean-Guy
Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire de Bioénergétique Cellulaire, CEA Cadarache, Bât 156, 13108 Saint-Paul Lez Durance, France.
Pierrugues Olivier
Brutesco Catherine
Alonso Béatrice
Montillet Jean-Luc
Roby Dominique
Kazmaier Michael
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-005-1113-1
Published
2005-03-00
Epub
2005-00-15
Pages
10-9
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-005-1113-1 View journal details
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