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PMID: 15269331 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vascular associated death1, a novel GRAM domain-containing protein, is a regulator of cell death and defense responses in vascular tissues.

The Plant cell ·Vol. 16 ·No. 8 ·2004-08-00 ·Pages 2217-32

Lorrain S, Lin B, Auriac MC, Kroj T, Saindrenan P, Nicole M, Balagué C, Roby D

Abstract

The hypersensitive response (HR) is a programmed cell death that is commonly associated with plant disease resistance. A novel lesion mimic mutant, vad1 (for vascular associated death1), that exhibits light conditional appearance of propagative HR-like lesions along the vascular system was identified. Lesion formation is associated with expression of defense genes, production of high levels of salicylic acid (SA), and increased resistance to virulent and avirulent strains of Pseudomonas syringae pv tomato. Analyses of the progeny from crosses between vad1 plants and either nahG transgenic plants, sid1, nonexpressor of PR1 (npr1), enhanced disease susceptibility1 (eds1), or non-race specific disease resistance1 (ndr1) mutants, revealed the vad1 cell death phenotype to be dependent on SA biosynthesis but NPR1 independent; in addition, both EDS1 and NDR1 are necessary for the proper timing and amplification of cell death as well as for increased resistance to Pseudomonas strains. VAD1 encodes a novel putative membrane-associated protein containing a GRAM domain, a lipid or protein binding signaling domain, and is expressed in response to pathogen infection at the vicinity of the hypersensitive lesions. VAD1 might thus represent a new potential function in cell death control associated with cells in the vicinity of vascular bundles.

MeSH Terms
Apoptosis/physiology Arabidopsis/anatomy & histology,microbiology,physiology Arabidopsis Proteins/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Plant Immunity, Innate/physiology Membrane Proteins/genetics,metabolism Mutation Phenotype Plant Diseases Plants, Genetically Modified Protein Structure, Tertiary Proteins/genetics,metabolism Pseudomonas syringae/pathogenicity Salicylic Acid/metabolism Transcription Factors
Chemicals
Arabidopsis Proteins DNA-Binding Proteins EDS1 protein, Arabidopsis Membrane Proteins NDR1 protein, Arabidopsis Proteins Transcription Factors Salicylic Acid
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lorrain Séverine
Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique 2594, Boîte Postale 27, 31326 Castanet-Tolosan, Cedex, France.
Lin Baiqing
Auriac Marie Christine
Kroj Thomas
Saindrenan Patrick
Nicole Michel
Balagué Claudine
Roby Dominique
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.022038
Published
2004-08-00
Epub
2004-00-21
Pages
2217-32
Language
English
Region
England
NLM ID
9208688
PMCID
PMC519209
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.104.022038 PMC Full Text View journal details
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