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PMID: 12034892 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis SGT1b is required for defense signaling conferred by several downy mildew resistance genes.

The Plant cell ·Vol. 14 ·No. 5 ·2002-05-00 ·Pages 993-1003

Tör M, Gordon P, Cuzick A, Eulgem T, Sinapidou E, Mert-Türk F, Can C, Dangl JL, Holub EB

Abstract

We describe the identification of a mutant in the Arabidopsis accession Columbia (Col-0) that exhibits enhanced downy mildew (edm1) susceptibility to several Peronospora parasitica isolates, including the RPP7-diagnostic isolate Hiks1. The mutation was mapped to chromosome IV and characterized physically as a 35-kb deletion spanning seven genes. One of these genes complemented the mutant to full wild-type resistance against all of the Peronospora isolates tested. This gene (AtSGT1b) encodes a predicted protein of 39.8 kD and is an Arabidopsis ortholog of yeast SGT1, which was described originally as a key regulatory protein in centromere function and ubiquitin-mediated proteolysis. AtSGT1b contains three tetratricopeptide repeats at the N terminus followed by a bipartite chord-containing SGT domain and an SGT-specific domain at the C terminus. We discuss the role of AtSGT1b in disease resistance and its possible involvement in ubiquitin-mediated proteolysis in plants.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,growth & development,microbiology Arabidopsis Proteins/genetics Cell Cycle Proteins/genetics Chromosome Mapping Cloning, Molecular Cotyledon/genetics,growth & development,microbiology Fungi/growth & development Gene Expression Regulation, Plant Genetic Complementation Test Immunity, Innate/genetics Molecular Sequence Data Mutation Phenotype Plant Diseases/genetics,microbiology Plants, Genetically Modified Sequence Homology, Amino Acid Signal Transduction/genetics Yeasts/genetics,growth & development
Chemicals
Arabidopsis Proteins Cell Cycle Proteins SGT1b protein, Arabidopsis
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Tör Mahmut
Plant Genetics and Biotechnology Department, Horticulture Research International, Wellesbourne, Warwick CV35 9EF, United Kingdom.
Gordon Pam
Cuzick Alayne
Eulgem Thomas
Sinapidou Evaggelia
Mert-Türk Figen
Can Canan
Dangl Jeffery L
Holub Eric B
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.001123
Published
2002-05-00
Pages
993-1003
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150602
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.001123 PMC Full Text View journal details
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