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PMID: 15699213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo.

The Journal of cell biology ·Vol. 168 ·No. 4 ·2005-02-14 ·Pages 575-85

Padash Barmchi M, Rogers S, Häcker U

Abstract

Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors.

MeSH Terms
Actins/metabolism Actomyosin/physiology Animals Blastoderm/cytology,metabolism Cell Cycle Proteins Cell Division/physiology Cell Polarity/physiology Cell Shape/physiology Cytoskeleton/physiology Drosophila Proteins/metabolism Drosophila melanogaster/embryology,genetics,metabolism Embryo, Nonmammalian/cytology,metabolism Embryonic Development/physiology Microfilament Proteins/metabolism Mutation/genetics rho GTP-Binding Proteins/metabolism
Chemicals
Actins Cell Cycle Proteins Drosophila Proteins Microfilament Proteins bnk protein, Drosophila sav protein, Drosophila Actomyosin rho GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Padash Barmchi Mojgan
Department of Cell and Molecular Biology, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, BMC B13, 22184 Lund, Sweden.
Rogers Stephen
Häcker Udo
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200407124
Published
2005-02-14
Epub
2005-00-07
Pages
575-85
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171764
Subset
IM
Grants
NIGMS NIH HHS · F32 GM064966 · United States
NIGMS NIH HHS · 5F32GM064966 · United States
External Links
PubMed source DOI: 10.1083/jcb.200407124 PMC Full Text View journal details
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