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PMID: 14676307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Balancing different types of actin polymerization at distinct sites: roles for Abelson kinase and Enabled.

The Journal of cell biology ·Vol. 163 ·No. 6 ·2003-12-22 ·Pages 1267-79

Grevengoed EE, Fox DT, Gates J, Peifer M

Abstract

The proto-oncogenic kinase Abelson (Abl) regulates actin in response to cell signaling. Drosophila Abl is required in the nervous system, and also in epithelial cells, where it regulates adherens junction stability and actin organization. Abl acts at least in part via the actin regulator Enabled (Ena), but the mechanism by which Abl regulates Ena is unknown. We describe a novel role for Abl in early Drosophila development, where it regulates the site and type of actin structures produced. In Abl's absence, excess actin is polymerized in apical microvilli, whereas too little actin is assembled into pseudocleavage and cellularization furrows. These effects involve Ena misregulation. In abl mutants, Ena accumulates ectopically at the apical cortex where excess actin is observed, suggesting that Abl regulates Ena's subcellular localization. We also examined other actin regulators. Loss of Abl leads to changes in the localization of the Arp2/3 complex and the formin Diaphanous, and mutations in diaphanous or capping protein beta enhance abl phenotypes.

MeSH Terms
Actin Depolymerizing Factors Actin-Related Protein 2 Actins/genetics,metabolism Animals Caenorhabditis elegans Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Cell Differentiation/genetics DNA-Binding Proteins/genetics,metabolism Destrin Drosophila Proteins Drosophila melanogaster/cytology,embryology,metabolism Embryo, Nonmammalian/cytology,embryology,metabolism Formins Gene Expression Regulation, Developmental/genetics Microfilament Proteins/genetics,metabolism Microvilli/metabolism,ultrastructure Mutation/genetics Phenotype Protein Transport/physiology Proto-Oncogene Proteins c-abl/genetics,metabolism
Chemicals
Actin Depolymerizing Factors Actin-Related Protein 2 Actins Caenorhabditis elegans Proteins Carrier Proteins DNA-Binding Proteins Destrin Drosophila Proteins ENA-VASP proteins Formins Microfilament Proteins arx-2 protein, C elegans diaphanous protein, Drosophila Proto-Oncogene Proteins c-abl
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grevengoed Elizabeth E
University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA.
Fox Donald T
Gates Julie
Peifer Mark
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39 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200307026
Published
2003-12-22
Epub
2003-00-15
Pages
1267-79
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173720
Subset
IM
Grants
NIGMS NIH HHS · R01 GM047857 · United States
NIGMS NIH HHS · 5T32GM07092 · United States
NCI NIH HHS · 1T32CA72319 · United States
NCI NIH HHS · T32 CA072319 · United States
NIGMS NIH HHS · T32 GM007092 · United States
NIGMS NIH HHS · F32 GM068337 · United States
NIGMS NIH HHS · GM47857 · United States
NIGMS NIH HHS · 1F32GM068337 · United States
External Links
PubMed source DOI: 10.1083/jcb.200307026 PMC Full Text View journal details
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