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PMID: 15686594 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium.

BMC infectious diseases ·Vol. 5 ·2005-02-01 ·Pages 6

Moës E, Vijgen L, Keyaerts E, Zlateva K, Li S, Maes P, Pyrc K, Berkhout B, van der Hoek L, Van Ranst M

Abstract

Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium. Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay. Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004. Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.

MeSH Terms
Adolescent Base Sequence Belgium/epidemiology Child Child, Preschool Consensus Sequence Coronavirus/genetics,isolation & purification Coronavirus Infections/epidemiology,virology DNA Primers/chemistry Female Humans Incidence Infant Male Molecular Sequence Data Phylogeny RNA, Viral/chemistry Respiratory Tract Infections/epidemiology,virology Reverse Transcriptase Polymerase Chain Reaction/methods Seasons Sequence Alignment
Chemicals
DNA Primers RNA, Viral
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Moës Elien
Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research, University of Leuven, Belgium. elien.moes@uz.kuleuven.ac.be
Vijgen Leen
Keyaerts Els
Zlateva Kalina
Li Sandra
Maes Piet
Pyrc Krzysztof
Berkhout Ben
van der Hoek Lia
Van Ranst Marc
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Article Info
Journal
BMC infectious diseases
Abbr.
BMC Infect Dis
ISSN
1471-2334
DOI
10.1186/1471-2334-5-6
Email
elien.moes@uz.kuleuven.ac.be
Published
2005-02-01
Epub
2005-00-01
Pages
6
Language
English
Region
England
NLM ID
100968551
PMCID
PMC549190
Subset
IM
Databases
GENBANK
AY758276, AY758277, AY758278, AY758279, AY758280, AY758281, AY758282, AY758283, AY758284, AY758285, AY758286, AY758287, AY758288, AY758289, AY758290, AY758291, AY758292, AY758293, AY758294, AY758295, AY758296, AY758297, AY758298, AY758299, AY758300, AY758301
External Links
PubMed source DOI: 10.1186/1471-2334-5-6 PMC Full Text View journal details
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