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PMID: 15681342 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

BZR1 is a transcriptional repressor with dual roles in brassinosteroid homeostasis and growth responses.

Science (New York, N.Y.) ·Vol. 307 ·No. 5715 ·2005-03-11 ·Pages 1634-8

He JX, Gendron JM, Sun Y, Gampala SS, Gendron N, Sun CQ, Wang ZY

Abstract

Brassinosteroid (BR) homeostasis and signaling are crucial for normal growth and development of plants. BR signaling through cell-surface receptor kinases and intracellular components leads to dephosphorylation and accumulation of the nuclear protein BZR1. How BR signaling regulates gene expression, however, remains unknown. Here we show that BZR1 is a transcriptional repressor that has a previously unknown DNA binding domain and binds directly to the promoters of feedback-regulated BR biosynthetic genes. Microarray analyses identified additional potential targets of BZR1 and illustrated, together with physiological studies, that BZR1 coordinates BR homeostasis and signaling by playing dual roles in regulating BR biosynthesis and downstream growth responses.

MeSH Terms
Arabidopsis/genetics,growth & development,physiology Arabidopsis Proteins/genetics,metabolism Base Sequence Binding Sites Chromatin Immunoprecipitation DNA-Binding Proteins/genetics,metabolism Feedback, Physiological Gene Expression Regulation, Plant Genes, Plant Genes, Reporter Homeostasis Light Mutation Nuclear Proteins/genetics,metabolism Oligonucleotide Array Sequence Analysis Phenotype Plant Growth Regulators/biosynthesis,metabolism,pharmacology Plants, Genetically Modified Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Repressor Proteins/metabolism Signal Transduction Steroids/biosynthesis,metabolism,pharmacology Transcription, Genetic
Chemicals
Arabidopsis Proteins BZR1 protein, Arabidopsis DNA-Binding Proteins Nuclear Proteins Plant Growth Regulators Recombinant Fusion Proteins Repressor Proteins Steroids
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
He Jun-Xian
Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA.
Gendron Joshua M
Sun Yu
Gampala Srinivas S L
Gendron Nathan
Sun Catherine Qing
Wang Zhi-Yong
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1107580
Published
2005-03-11
Epub
2005-00-27
Pages
1634-8
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2925132
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM007276 · United States
NIGMS NIH HHS · R01 GM66258-01 · United States
NIGMS NIH HHS · T32 GM007276 · United States
NIGMS NIH HHS · R01 GM066258-04 · United States
NIGMS NIH HHS · R01 GM066258 · United States
Corrections
ErratumIn
-
CommentIn
PMID: 15761142
External Links
PubMed source DOI: 10.1126/science.1107580 PMC Full Text View journal details
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