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PMID: 14742873 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Aux/IAA proteins contain a potent transcriptional repression domain.

The Plant cell ·Vol. 16 ·No. 2 ·2004-02-00 ·Pages 533-43

Tiwari SB, Hagen G, Guilfoyle TJ

Abstract

Aux/IAA proteins are short-lived nuclear proteins that repress expression of primary/early auxin response genes in protoplast transfection assays. Repression is thought to result from Aux/IAA proteins dimerizing with auxin response factor (ARF) transcriptional activators that reside on auxin-responsive promoter elements, referred to as AuxREs. Most Aux/IAA proteins contain four conserved domains, designated domains I, II, III, and IV. Domain II and domains III and IV play roles in protein stability and dimerization, respectively. A clear function for domain I had not been established. Results reported here indicate that domain I in Aux/IAA proteins is an active repression domain that is transferable and dominant over activation domains. An LxLxL motif within domain I is important for conferring repression. The dominance of Aux/IAA repression domains over activation domains in ARF transcriptional activators provides a plausible explanation for the repression of auxin response genes via ARF-Aux/IAA dimerization on auxin-responsive promoters.

MeSH Terms
Amino Acid Sequence Arabidopsis Proteins/chemistry,genetics,metabolism Binding Sites/genetics DNA-Binding Proteins Leucine/metabolism Nuclear Proteins/chemistry,genetics,metabolism Plant Proteins Protein Interaction Mapping Recombinant Fusion Proteins/genetics,metabolism Repressor Proteins/genetics,metabolism Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
AXR3 protein, Arabidopsis Arabidopsis Proteins DNA-Binding Proteins Nuclear Proteins Plant Proteins Recombinant Fusion Proteins Repressor Proteins Trans-Activators Transcription Factors superman protein, Arabidopsis ethylene-responsive element binding protein Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tiwari Shiv B
Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA.
Hagen Gretchen
Guilfoyle Tom J
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32 references, click to expand
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.017384
Published
2004-02-00
Epub
2004-00-23
Pages
533-43
Language
English
Region
England
NLM ID
9208688
PMCID
PMC341922
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.017384 PMC Full Text View journal details
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