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PMID: 15650748 Published · ppublish English Journal Article

Elf5 is essential for early embryogenesis and mammary gland development during pregnancy and lactation.

The EMBO journal ·Vol. 24 ·No. 3 ·2005-02-09 ·Pages 635-44

Zhou J, Chehab R, Tkalcevic J, Naylor MJ, Harris J, Wilson TJ, Tsao S, Tellis I, Zavarsek S, Xu D, Lapinskas EJ, Visvader J, Lindeman GJ, Thomas R, Ormandy CJ, Hertzog PJ, Kola I, Pritchard MA

Abstract

Elf5 is an epithelial-specific ETS factor. Embryos with a null mutation in the Elf5 gene died before embryonic day 7.5, indicating that Elf5 is essential during mouse embryogenesis. Elf5 is also required for proliferation and differentiation of mouse mammary alveolar epithelial cells during pregnancy and lactation. The loss of one functional allele led to complete developmental arrest of the mammary gland in pregnant Elf5 heterozygous mice. A quantitative mRNA expression study and Western blot analysis revealed that decreased expression of Elf5 correlated with the downregulation of milk proteins in Elf5(+/-) mammary glands. Mammary gland transplants into Rag(-/-) mice demonstrated that Elf5(+/-) mammary alveolar buds failed to develop in an Elf5(+/+) mammary fat pad during pregnancy, demonstrating an epithelial cell autonomous defect. Elf5 expression was reduced in Prolactin receptor (Prlr) heterozygous mammary glands, which phenocopy Elf5(+/-) glands, suggesting that Elf5 and Prlr are in the same pathway. Our data demonstrate that Elf5 is essential for developmental processes in the embryo and in the mammary gland during pregnancy.

MeSH Terms
Animals Base Sequence DNA/genetics Epithelial Cells/pathology Female Fetal Development/genetics,physiology Gene Expression Regulation, Developmental Heterozygote Lactation Mammary Glands, Animal/growth & development,pathology Mice Mice, Knockout Pregnancy RNA, Messenger/genetics,metabolism Receptors, Prolactin/genetics,metabolism Transcription Factors/deficiency,genetics,physiology
Chemicals
RNA, Messenger Receptors, Prolactin Transcription Factors DNA
Authors & Affiliations
18 authors, click to expand affiliations / ORCID
Zhou Jiong
Centre for Functional Genomics and Human Disease, Monash Institute of Reproduction and Development, Monash University, Melbourne, Victoria, Australia.
Chehab Renee
Tkalcevic Josephine
Naylor Matthew J
Harris Jessica
Wilson Trevor J
Tsao Sue
Tellis Irene
Zavarsek Silva
Xu Dakang
Lapinskas Erika J
Visvader Jane
Lindeman Geoffrey J
Thomas Ross
Ormandy Christopher J
Hertzog Paul J
Kola Ismail
Pritchard Melanie A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600538
Published
2005-02-09
Epub
2005-00-13
Pages
635-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC548648
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600538 PMC Full Text View journal details
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