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PMID: 15616194 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Opposing roles for actin in Cdc42p polarization.

Molecular biology of the cell ·Vol. 16 ·No. 3 ·2005-03-00 ·Pages 1296-304

Irazoqui JE, Howell AS, Theesfeld CL, Lew DJ

Abstract

In animal and fungal cells, the monomeric GTPase Cdc42p is a key regulator of cell polarity that itself exhibits a polarized distribution in asymmetric cells. Previous work showed that in budding yeast, Cdc42p polarization is unaffected by depolymerization of the actin cytoskeleton (Ayscough et al., J. Cell Biol. 137, 399-416, 1997). Surprisingly, we now report that unlike complete actin depolymerization, partial actin depolymerization leads to the dispersal of Cdc42p from the polarization site in unbudded cells. We provide evidence that dispersal is due to endocytosis associated with cortical actin patches and that actin cables are required to counteract the dispersal and maintain Cdc42p polarity. Thus, although Cdc42p is initially polarized in an actin-independent manner, maintaining that polarity may involve a reinforcing feedback between Cdc42p and polarized actin cables to counteract the dispersing effects of actin-dependent endocytosis. In addition, we report that once a bud has formed, polarized Cdc42p becomes more resistant to dispersal, revealing an unexpected difference between unbudded and budded cells in the organization of the polarization site.

MeSH Terms
Actins/chemistry,physiology Binding Sites Cytoskeleton/metabolism Endocytosis Genotype Microscopy, Fluorescence Models, Biological Mutation Protein Binding Saccharomyces cerevisiae/metabolism,physiology Temperature Time Factors cdc42 GTP-Binding Protein/chemistry
Chemicals
Actins cdc42 GTP-Binding Protein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Irazoqui Javier E
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.
Howell Audrey S
Theesfeld Chandra L
Lew Daniel J
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-05-0430
Published
2005-03-00
Epub
2004-00-22
Pages
1296-304
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC551493
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062300 · United States
NIGMS NIH HHS · GM63200 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-05-0430 PMC Full Text View journal details
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