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PMID: 15604146 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of a proteolytically resistant core from the severe acute respiratory syndrome coronavirus S2 fusion protein.

Supekar VM, Bruckmann C, Ingallinella P, Bianchi E, Pessi A, Carfí A

Abstract

A coronavirus (CoV) has recently been identified as the causative agent of the severe acute respiratory syndrome (SARS) in humans. CoVs enter target cells through fusion of viral and cellular membranes mediated by the viral envelope glycoprotein S. We have determined by x-ray crystallography the structure of a proteolytically stable core fragment from the heptad repeat (HR) regions HR1 and HR2 of the SARS-CoV S protein. We have also determined the structure of an HR1-HR2 S core fragment, containing a shorter HR1 peptide and a C-terminally longer HR2 peptide that extends up to the transmembrane region. In these structures, three HR1 helices form a parallel coiled-coil trimer, whereas three HR2 peptides pack in an oblique and antiparallel fashion into the coiled-coil hydrophobic grooves, adopting mixed extended and alpha-helical conformations as in postfusion paramyxoviruses F proteins structures. Our structure positions a previously proposed internal fusion peptide adjacent to the N-terminus of HR1. Peptides from the HR2 region of SARS-CoV S have been shown to inhibit viral entry and infection in vitro. The structures presented here can thus open the path to the design of small-molecule inhibitors of viral entry and candidate vaccine antigens against this virus.

MeSH Terms
Amino Acid Sequence Antigens, Viral/chemistry Cell Membrane/metabolism Conserved Sequence Crystallography, X-Ray Dimerization Membrane Glycoproteins/chemistry,metabolism Models, Molecular Molecular Sequence Data Mutation Paramyxoviridae/metabolism Peptides/chemistry Protein Conformation Protein Structure, Secondary Protein Structure, Tertiary Sequence Homology, Amino Acid Spike Glycoprotein, Coronavirus Viral Envelope Proteins/chemistry,metabolism Viral Fusion Proteins/chemistry
Chemicals
Antigens, Viral Membrane Glycoproteins Peptides Spike Glycoprotein, Coronavirus Viral Envelope Proteins Viral Fusion Proteins spike glycoprotein, SARS-CoV spike protein, mouse hepatitis virus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Supekar Vinit M
Istituto di Ricerche di Biologia Molecolare P. Angeletti, Via Pontina Km 30,600, 00040 Pomezia (Rome), Italy.
Bruckmann Chiara
Ingallinella Paolo
Bianchi Elisabetta
Pessi Antonello
Carfí Andrea
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
2004-12-28
Epub
2004-00-16
Pages
17958-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC539766
Subset
IM
Databases
PDB
Analysis Services
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