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PMID: 1557383 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Partial characterization of vertebrate prothrombin cDNAs: amplification and sequence analysis of the B chain of thrombin from nine different species.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 7 ·1992-04-01 ·Pages 2779-83

Banfield DK, MacGillivray RT

Abstract

The cDNA sequence of the B chain of thrombin (EC 3.4.21.5) has been determined from nine vertebrate species (rat, mouse, rabbit, chicken, gecko, newt, rainbow trout, sturgeon, and hagfish). The amino acid sequence identities vary from 96.5% (rat vs. mouse) to 62.6% (newt vs. hagfish). Of the 240 amino acids spanned in all the species compared, there is identity at 110 (45.8%) positions. When conservative changes are included, the amino acid similarity increases to 75%. The most conserved portions of the B chain are the active-site residues and adjacent amino acids, the B loop, and the primary substrate-binding region. In addition, the Arg-Gly-Asp motif is conserved in 9 of the 11 species compared, and the chemotactic/growth factor domain is well conserved in all of the 11 species compared. The least conserved regions of the B chain correspond to surface loops, including the putative thrombomodulin-binding sites and one of the hirudin-binding regions. The extent of the amino acid sequence similarity and the conservation of many of the functional/structural motifs suggests that, in addition to their role in blood coagulation, vertebrate thrombins may also play an important role in the general mechanisms of wound repair.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens DNA/genetics Fishes Lizards Mice Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Polymerase Chain Reaction Prothrombin/genetics Rabbits Rats Salamandridae Sequence Alignment
Chemicals
Oligodeoxyribonucleotides Prothrombin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Banfield D K
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
MacGillivray R T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.7.2779
Published
1992-04-01
Pages
2779-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48746
Subset
IM
Databases
GENBANK
M81391, M81392, M81393, M81394, M81395, M81396, M81397, M81398, M81399
External Links
PubMed source DOI: 10.1073/pnas.89.7.2779 PMC Full Text View journal details
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