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PMID: 15548607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transplanted human bone marrow contributes to vascular endothelium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 48 ·2004-11-30 ·Pages 16891-6

Jiang S, Walker L, Afentoulis M, Anderson DA, Jauron-Mills L, Corless CL, Fleming WH

Abstract

Recent evidence indicates that bone marrow is a source of endothelial progenitor cells that are mobilized into the peripheral blood in response to cytokines or tissue injury. Previously, we showed that functional endothelial cells (ECs) can be clonally derived from phenotypically defined hematopoietic stem cells. To determine the EC potential of human bone marrow and peripheral blood stem cells, blood vessels in sex-mismatched transplant recipients were evaluated. EC outcomes were identified by using a combination of immunohistochemistry and XY interphase FISH. Donor-derived ECs were detected in the skin and gut of transplant recipients with a mean frequency of 2% and could readily be distinguished from CD45-expressing hematopoietic stem cells. None of the >4,000 ECs examined had more than two sex chromosomes, consistent with an absence of cell fusion. Y chromosome signals were not detected in sex-matched female recipients, excluding the vertical transmission of male cells. None of the recipients evaluated before hematopoietic engraftment demonstrated donor-derived ECs, indicating a close linkage between the recovery of hematopoiesis and EC outcomes. Transplantable bone marrow-derived endothelial progenitor cells may represent novel therapeutic targets for hematopoietic and vascular disease.

MeSH Terms
Biopsy Bone Marrow Transplantation Case-Control Studies Diploidy Endothelium, Vascular/cytology Female Humans In Situ Hybridization, Fluorescence Intestinal Mucosa/cytology Male Skin/pathology
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jiang Shuguang
Center for Hematologic Malignancies, Division of Hematology and Medical Oncology, Cancer Institute, Oregon Health and Science University, Portland, OR 97239, USA.
Walker Luke
Afentoulis Michael
Anderson Daniel A
Jauron-Mills Linda
Corless Christopher L
Fleming William H
References (44)
44 references, click to expand
  1. Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation.
    Hepatology. 2000 Jan;31(1):235-40 PMID: 10613752
  2. A few thoughts about the plasticity of stem cells.
    Exp Hematol. 2002 Aug;30(8):848-52 PMID: 12160835
  3. Liver from bone marrow in humans.
    Hepatology. 2000 Jul;32(1):11-6 PMID: 10869283
  4. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo.
    Nat Med. 2000 Nov;6(11):1229-34 PMID: 11062533
  5. Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow.
    Science. 2000 Dec 1;290(5497):1779-82 PMID: 11099419
  6. Selective inhibition of vascular endothelial growth factor-mediated angiogenesis by cyclosporin A: roles of the nuclear factor of activated T cells and cyclooxygenase 2.
    J Exp Med. 2001 Mar 5;193(5):607-20 PMID: 11238591
  7. Bone marrow cells regenerate infarcted myocardium.
    Nature. 2001 Apr 5;410(6829):701-5 PMID: 11287958
  8. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell.
    Cell. 2001 May 4;105(3):369-77 PMID: 11348593
  9. Targeting gene-modified hematopoietic cells to the central nervous system: use of green fluorescent protein uncovers microglial engraftment.
    Nat Med. 2001 Dec;7(12):1356-61 PMID: 11726978
  10. Origin of endothelial progenitors in human postnatal bone marrow.
    J Clin Invest. 2002 Feb;109(3):337-46 PMID: 11827993
  11. Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells.
    N Engl J Med. 2002 Mar 7;346(10):738-46 PMID: 11882729
  12. Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization.
    Nat Med. 2002 Jun;8(6):607-12 PMID: 12042812
  13. Damaged epithelia regenerated by bone marrow-derived cells in the human gastrointestinal tract.
    Nat Med. 2002 Sep;8(9):1011-7 PMID: 12195435
  14. Little evidence for developmental plasticity of adult hematopoietic stem cells.
    Science. 2002 Sep 27;297(5590):2256-9 PMID: 12215650
  15. Identification of the hemangioblast in postnatal life.
    Blood. 2002 Nov 1;100(9):3203-8 PMID: 12384418
  16. Transplanted bone marrow generates new neurons in human brains.
    Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1364-9 PMID: 12538864
  17. Contribution of transplanted bone marrow cells to Purkinje neurons in human adult brains.
    Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2088-93 PMID: 12576546
  18. Hematopoietic origin of glomerular mesangial cells.
    Blood. 2003 Mar 15;101(6):2215-8 PMID: 12433693
  19. Differentiation, cell fusion, and nuclear fusion during ex vivo repair of epithelium by human adult stem cells from bone marrow stroma.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2397-402 PMID: 12606728
  20. Mixed chimerism of bone marrow vessels (endothelial cells, myofibroblasts) following allogeneic transplantation for chronic myelogenous leukemia.
    Leuk Lymphoma. 2003 Feb;44(2):321-8 PMID: 12688352
  21. Cell fusion is the principal source of bone-marrow-derived hepatocytes.
    Nature. 2003 Apr 24;422(6934):897-901 PMID: 12665832
  22. Human pulmonary chimerism after hematopoietic stem cell transplantation.
    Am J Respir Crit Care Med. 2003 Aug 1;168(3):318-22 PMID: 12724127
  23. Converging roads: evidence for an adult hemangioblast.
    Exp Hematol. 2003 Nov;31(11):987-93 PMID: 14585360
  24. Single hematopoietic stem cells generate skeletal muscle through myeloid intermediates.
    Nat Med. 2003 Dec;9(12):1520-7 PMID: 14625546
  25. Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells.
    Blood. 2004 Jan 1;103(1):13-9 PMID: 12958072
  26. Skeletal muscle fiber-specific green autofluorescence: potential for stem cell engraftment artifacts.
    Stem Cells. 2004;22(2):180-7 PMID: 14990857
  27. Epithelial tissue chimerism after human hematopoietic cell transplantation is a real phenomenon.
    Am J Pathol. 2004 Apr;164(4):1147-55 PMID: 15039204
  28. Bone marrow transdifferentiation in brain after transplantation: a retrospective study.
    Lancet. 2004 May 1;363(9419):1432-7 PMID: 15121406
  29. Bone marrow-derived hematopoietic cells generate cardiomyocytes at a low frequency through cell fusion, but not transdifferentiation.
    Nat Med. 2004 May;10(5):494-501 PMID: 15107841
  30. Myelomonocytic cells are sufficient for therapeutic cell fusion in liver.
    Nat Med. 2004 Jul;10(7):744-8 PMID: 15195088
  31. A phase and electron microscopic study of vasculogenesis and erythropoiesis in the yolk sac of the mouse.
    Anat Rec. 1971 Jun;170(2):199-223 PMID: 4931266
  32. Bone marrow origin of hepatic macrophages (Kupffer cells) in humans.
    Science. 1978 Sep 8;201(4359):937-8 PMID: 356266
  33. Brief report: donor-derived long-term multilineage hematopoiesis in a liver-transplant recipient.
    N Engl J Med. 1993 Mar 18;328(11):762-5 PMID: 8094887
  34. Male donor-derived cells in the brains of female sex-mismatched bone marrow transplant recipients: a Y-chromosome specific in situ hybridization study.
    J Neuropathol Exp Neurol. 1993 Sep;52(5):460-70 PMID: 8103085
  35. Fluorescence in situ hybridization with X and Y DNA specific probes for chimerism detection in pulmonary alveolar macrophages after human sex-mismatched allogeneic bone marrow transplantation.
    Bone Marrow Transplant. 1994 Jul;14(1):177-9 PMID: 7951114
  36. Aorta-associated CD34+ hematopoietic cells in the early human embryo.
    Blood. 1996 Jan 1;87(1):67-72 PMID: 8547678
  37. Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum.
    Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):705-8 PMID: 8570620
  38. Purification and expansion of human hematopoietic stem/progenitor cells.
    Ann N Y Acad Sci. 1995 Dec 29;770:91-8 PMID: 8597386
  39. Muscle regeneration by bone marrow-derived myogenic progenitors.
    Science. 1998 Mar 6;279(5356):1528-30 PMID: 9488650
  40. Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization.
    Nat Med. 1999 Apr;5(4):434-8 PMID: 10202935
  41. Survival of donor leukocyte subpopulations in immunocompetent transfusion recipients: frequent long-term microchimerism in severe trauma patients.
    Blood. 1999 May 1;93(9):3127-39 PMID: 10216112
  42. Recruitment of bone-marrow-derived cells by skeletal and cardiac muscle in adult dystrophic mdx mice.
    Anat Embryol (Berl). 1999 May;199(5):391-6 PMID: 10221450
  43. Can stem cells cross lineage boundaries?
    Nat Med. 2001 Apr;7(4):393-5 PMID: 11283651
  44. Origins of circulating endothelial cells and endothelial outgrowth from blood.
    J Clin Invest. 2000 Jan;105(1):71-7 PMID: 10619863
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0404398101
Published
2004-11-30
Epub
2004-00-17
Pages
16891-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC534718
Subset
IM
Grants
NICHD NIH HHS · R01 HD039251 · United States
NHLBI NIH HHS · R01 HL069133 · United States
NICHD NIH HHS · HD 39251 · United States
NHLBI NIH HHS · HL 069133 · United States
External Links
PubMed source DOI: 10.1073/pnas.0404398101 PMC Full Text View journal details
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