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PMID: 15509735 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Neurofilament transport is dependent on actin and myosin.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 24 ·No. 43 ·2004-10-27 ·Pages 9486-96

Jung C, Chylinski TM, Pimenta A, Ortiz D, Shea TB

Abstract

Real-time analyses have revealed that some newly synthesized neurofilament (NF) subunits translocate into and along axonal neurites by moving along the inner plasma membrane surface, suggesting that they may translocate against the submembrane actin cortex. We therefore examined whether or not NF axonal transport was dependent on actin and myosin. Perturbation of filamentous actin in NB2a/d1 cells with cytochalasin B inhibited translocation of subunits into axonal neurites and inhibited bidirectional translocation of NF subunits within neurites. Intravitreal injection of cytochalasin B inhibited NF axonal transport in optic axons in a dose-response manner. NF subunits were coprecipitated from NB2a/d1 cells by an anti-myosin antibody, and myosin colocalized with NFs in immunofluorescent analyses. The myosin light chain kinase inhibitor ML-7 and the myosin ATPase inhibitor 2,3-butanedione-2-monoxime perturbed NF translocation within NB2a/d1 axonal neurites. These findings suggest that some NF subunits may undergo axonal transport via myosin-mediated interactions with the actin cortex.

MeSH Terms
Actins/drug effects,physiology Animals Axonal Transport/drug effects,physiology Azepines/pharmacology Cell Line, Tumor Cytochalasin B/pharmacology Diacetyl/analogs & derivatives,pharmacology Green Fluorescent Proteins Mice Myosin-Light-Chain Kinase/antagonists & inhibitors Myosins/antagonists & inhibitors,physiology Naphthalenes/pharmacology Neurites/ultrastructure Visual Pathways/drug effects,physiology,ultrastructure
Chemicals
Actins Azepines Naphthalenes ML 7 Green Fluorescent Proteins diacetylmonoxime Cytochalasin B Myosin-Light-Chain Kinase Myosins Diacetyl
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jung Cheolwha
Center for Cellular Neurobiology and Neurodegeneration Research, Departments of Biological Sciences and Biochemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854, USA.
Chylinski Teresa M
Pimenta Aurea
Ortiz Daniela
Shea Thomas B
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.1665-04.2004
Published
2004-10-27
Pages
9486-96
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6730143
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.1665-04.2004 PMC Full Text View journal details
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