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PMID: 11029051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bidirectional translocation of neurofilaments along microtubules mediated in part by dynein/dynactin.

Molecular biology of the cell ·Vol. 11 ·No. 10 ·2000-10-00 ·Pages 3495-508

Shah JV, Flanagan LA, Janmey PA, Leterrier JF

Abstract

Neuronal cytoskeletal elements such as neurofilaments, F-actin, and microtubules are actively translocated by an as yet unidentified mechanism. This report describes a novel interaction between neurofilaments and microtubule motor proteins that mediates the translocation of neurofilaments along microtubules in vitro. Native neurofilaments purified from spinal cord are transported along microtubules at rates of 100-1000 nm/s to both plus and minus ends. This motion requires ATP and is partially inhibited by vanadate, consistent with the activity of neurofilament-bound molecular motors. Motility is in part mediated by the dynein/dynactin motor complex and several kinesin-like proteins. This reconstituted motile system suggests how slow net movement of cytoskeletal polymers may be achieved by alternating activities of fast microtubule motors.

MeSH Terms
Animals Cattle Dynactin Complex Dyneins/isolation & purification,physiology Intermediate Filaments/physiology,ultrastructure Microtubule-Associated Proteins/isolation & purification,physiology Microtubules/physiology,ultrastructure Movement Neurons/physiology,ultrastructure Rats Spinal Cord/cytology
Chemicals
Dynactin Complex Microtubule-Associated Proteins Dyneins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shah J V
Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA.
Flanagan L A
Janmey P A
Leterrier J F
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.10.3495
Published
2000-10-00
Pages
3495-508
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC15009
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.11.10.3495 PMC Full Text View journal details
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