Home LiteratureArticle Details
PMID: 1546959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of a cDNA encoding the glycoprotein of hen oviduct microsomal signal peptidase.

The Biochemical journal ·Vol. 282 ( Pt 2) ·1992-03-01 ·Pages 447-52

Newsome AL, McLean JW, Lively MO

Abstract

Detergent-solubilized hen oviduct signal peptidase has been characterized previously as an apparent complex of a 19 kDa protein and a 23 kDa glycoprotein (GP23) [Baker & Lively (1987) Biochemistry 26, 8561-8567]. A cDNA clone encoding GP23 from a chicken oviduct lambda gt11 cDNA library has now been characterized. The cDNA encodes a protein of 180 amino acid residues with a single site for asparagine-linked glycosylation that has been directly identified by amino acid sequence analysis of a tryptic-digest peptide containing the glycosylated site. Immunoblot analysis reveals cross-reactivity with a dog pancreas protein. Comparison of the deduced amino acid sequence of GP23 with the 22/23 kDa glycoprotein of dog microsomal signal peptidase [Shelness, Kanwar & Blobel (1988) J. Biol. Chem. 263, 17063-17070], one of five proteins associated with this enzyme, reveals that the amino acid sequences are 90% identical. Thus the signal peptidase glycoprotein is as highly conserved as the sequences of cytochromes c and b from these same species and is likely to be found in a similar form in many, if not all, vertebrate species. The data also show conclusively that the dog and avian signal peptidases have at least one protein subunit in common.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Western Chickens Cloning, Molecular Cross Reactions DNA/genetics Dogs Endopeptidases/genetics Female Glycoproteins/genetics Membrane Proteins Microsomes/enzymology Molecular Sequence Data Oviducts/enzymology Sequence Homology, Nucleic Acid Serine Endopeptidases
Chemicals
Glycoproteins Membrane Proteins DNA Endopeptidases Serine Endopeptidases type I signal peptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Newsome A L
Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
McLean J W
Lively M O
References (43)
43 references, click to expand
  1. M13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2809-13 PMID: 6344069
  2. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  3. A bacterial secretory protein requires signal recognition particle for translocation across mammalian endoplasmic reticulum.
    J Biol Chem. 1982 Oct 25;257(20):11860-3 PMID: 6749848
  4. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
    Methods Enzymol. 1983;91:227-36 PMID: 6855576
  5. Expression and processing of bacterial beta-lactamase in the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4466-70 PMID: 7027263
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  8. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  9. The amino acid sequence of dog (Canis familiaris) hemoglobin.
    J Mol Evol. 1977 May 13;9(3):231-5 PMID: 864726
  10. Consensus sequence for processing of peptide precursors at monobasic sites.
    FEBS Lett. 1991 Mar 25;280(2):189-94 PMID: 2013311
  11. A subunit of mammalian signal peptidase is homologous to yeast SEC11 protein.
    J Biol Chem. 1989 Sep 25;264(27):15762-5 PMID: 2674115
  12. Signal peptidases in protein biosynthesis and intracellular transport.
    Curr Opin Cell Biol. 1989 Dec;1(6):1188-93 PMID: 2699805
  13. Mitochondrial protein import: identification of processing peptidase and of PEP, a processing enhancing protein.
    Cell. 1988 Jun 3;53(5):795-806 PMID: 2967109
  14. cDNA-derived primary structure of the glycoprotein component of canine microsomal signal peptidase complex.
    J Biol Chem. 1988 Nov 15;263(32):17063-70 PMID: 3053702
  15. Sequence and expression of a functional chicken progesterone receptor.
    Mol Endocrinol. 1987 Aug;1(8):517-25 PMID: 3153474
  16. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  17. Purification of microsomal signal peptidase as a complex.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):581-5 PMID: 3511473
  18. The detection and classification of membrane-spanning proteins.
    Biochim Biophys Acta. 1985 May 28;815(3):468-76 PMID: 3838905
  19. Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.
    J Mol Biol. 1985 May 5;183(1):1-12 PMID: 4009718
  20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  21. Aligning amino acid sequences: comparison of commonly used methods.
    J Mol Evol. 1984-1985;21(2):112-25 PMID: 6100188
  22. Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.
    J Biol Chem. 1983 Oct 10;258(19):12073-80 PMID: 6311837
  23. Isolation and characterization of two alleles of the chicken cytochrome c gene.
    Nucleic Acids Res. 1983 Dec 20;11(24):8931-50 PMID: 6324108
  24. Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3708-12 PMID: 6374664
  25. Preparation of microsomal membranes for cotranslational protein translocation.
    Methods Enzymol. 1983;96:84-93 PMID: 6656655
  26. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  27. Current problems in mechanistic studies of serine and cysteine proteinases.
    Biochem J. 1982 Oct 1;207(1):1-10 PMID: 6758764
  28. Purification and characterization of leader (signal) peptidase from Escherichia coli.
    J Biol Chem. 1980 Aug 25;255(16):7973-7 PMID: 6995457
  29. No evidence for 'stress' alpha-globin genes in chicken.
    Nature. 1982 Feb 25;295(5851):710-2 PMID: 7057930
  30. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  31. Tryptic dissection and reconstitution of translocation activity for nascent presecretory proteins across microsomal membranes.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1795-9 PMID: 109833
  32. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  33. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  34. Yeast signal peptidase contains a glycoprotein and the Sec11 gene product.
    Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):517-21 PMID: 1846444
  35. Two subunits of the canine signal peptidase complex are homologous to yeast SEC11 protein.
    J Biol Chem. 1990 Jun 5;265(16):9512-9 PMID: 2188978
  36. Purification and characterization of hen oviduct microsomal signal peptidase.
    Biochemistry. 1987 Dec 29;26(26):8561-7 PMID: 2831945
  37. Specific-primer-directed DNA sequencing.
    Anal Biochem. 1986 Apr;154(1):353-60 PMID: 3010776
  38. The processing peptidase of yeast mitochondria: the two co-operating components MPP and PEP are structurally related.
    EMBO J. 1988 Nov;7(11):3493-500 PMID: 3061797
  39. SEC11 is required for signal peptide processing and yeast cell growth.
    J Cell Biol. 1988 Apr;106(4):1035-42 PMID: 3283143
  40. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  41. Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F.
    Biochemistry. 1985 Aug 13;24(17):4665-71 PMID: 4063349
  42. Amino acid sequence of dog heart cytochrome c.
    J Biol Chem. 1965 Dec;240(12):4635-47 PMID: 5846985
  43. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2820447
Published
1992-03-01
Pages
447-52
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1130799
Subset
IM
Grants
NCI NIH HHS · CA 12197 · United States
NIGMS NIH HHS · GM 32861 · United States
NCRR NIH HHS · RR 04869 · United States
Databases
GENBANK
M57479, M86474, M86475, M86476, M86477, M86478, M86479, M86480, M86481, X60795
External Links
PubMed source DOI: 10.1042/bj2820447 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2