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PMID: 15466710 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The complete genomic sequence of Nocardia farcinica IFM 10152.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 41 ·2004-10-12 ·Pages 14925-30

Ishikawa J, Yamashita A, Mikami Y, Hoshino Y, Kurita H, Hotta K, Shiba T, Hattori M

Abstract

We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNF1) and 87,093 (pNF2) bp with average G+C contents of 67.2% and 68.4%, respectively. The chromosome encoded 5,674 putative protein-coding sequences, including many candidate genes for virulence and multidrug resistance as well as secondary metabolism. Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease.

MeSH Terms
Amino Acid Sequence Chromosome Mapping Chromosomes, Bacterial/genetics Genome, Bacterial Molecular Sequence Data Nocardia/genetics RNA Polymerase II/genetics Sequence Alignment Sequence Homology, Amino Acid
Chemicals
RNA Polymerase II
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ishikawa Jun
Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan. jun@nih.go.jp
Yamashita Atsushi
Mikami Yuzuru
Hoshino Yasutaka
Kurita Haruyo
Hotta Kunimoto
Shiba Tadayoshi
Hattori Masahira
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0406410101
Email
jun@nih.go.jp
Published
2004-10-12
Epub
2004-00-04
Pages
14925-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC522048
Subset
IM
Databases
GENBANK
AP006618, AP006619, AP006620
External Links
PubMed source DOI: 10.1073/pnas.0406410101 PMC Full Text View journal details
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