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PMID: 15466023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Flagellin from Listeria monocytogenes is glycosylated with beta-O-linked N-acetylglucosamine.

Journal of bacteriology ·Vol. 186 ·No. 20 ·2004-10-00 ·Pages 6721-7

Schirm M, Kalmokoff M, Aubry A, Thibault P, Sandoz M, Logan SM

Abstract

Glycan staining of purified flagellin from Listeria monocytogenes serotypes 1/2a, 1/2b, 1/2c, and 4b suggested that the flagellin protein from this organism is glycosylated. Mass spectrometry analysis demonstrated that the flagellin protein of L. monocytogenes is posttranslationally modified with O-linked N-acetylglucosamine (GlcNAc) at up to six sites/monomer. The sites of glycosylation are all located in the central, surface-exposed region of the protein monomer. Immunoblotting with a monoclonal antibody specific for beta-O-linked GlcNAc confirmed that the linkage was in the beta configuration, this residue being a posttranslational modification commonly observed in eukaryote nuclear and cytoplasmic proteins.

MeSH Terms
Acetylglucosamine/chemistry,metabolism Amino Acid Sequence Flagellin/chemistry,genetics,metabolism Glycosylation Listeria monocytogenes/genetics,metabolism Mass Spectrometry Molecular Sequence Data Polysaccharides/metabolism Protein Processing, Post-Translational
Chemicals
Polysaccharides Flagellin Acetylglucosamine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schirm M
Department of Chemistry, University of Montreal, Montreal, Quebec, Canada.
Kalmokoff M
Aubry A
Thibault P
Sandoz M
Logan S M
References (46)
46 references, click to expand
  1. Listeria monocytogenes Scott A: cell surface charge, hydrophobicity, and electron donor and acceptor characteristics under different environmental growth conditions.
    Appl Environ Microbiol. 1999 Dec;65(12):5328-33 PMID: 10583984
  2. Evidence for an heterogeneous glycosylation of the Clostridium tyrobutyricum ATCC 25755 flagellin.
    Microbios. 1998;94(379):183-92 PMID: 9853380
  3. Persistent Listeria monocytogenes strains show enhanced adherence to food contact surface after short contact times.
    J Food Prot. 2000 Sep;63(9):1204-7 PMID: 10983793
  4. Role of flm locus in mesophilic Aeromonas species adherence.
    Infect Immun. 2001 Jan;69(1):65-74 PMID: 11119490
  5. Crystallization of the F41 fragment of flagellin and data collection from extremely thin crystals.
    J Struct Biol. 2000 Nov;132(2):106-11 PMID: 11162732
  6. Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine.
    Anal Biochem. 2001 Jun 15;293(2):169-77 PMID: 11399029
  7. Characterization of a large motility gene cluster containing the cheR, motAB genes of Listeria monocytogenes and evidence that PrfA downregulates motility genes.
    FEMS Microbiol Lett. 1998 Dec 15;169(2):341-7 PMID: 9868779
  8. Characterization of Listeria monocytogenes from an ice cream plant by serotyping and pulsed-field gel electrophoresis.
    Int J Food Microbiol. 1999 Feb 18;46(3):187-92 PMID: 10100898
  9. Adherence of Listeria monocytogenes strains to stainless steel coupons.
    J Appl Microbiol. 1999 Apr;86(4):576-82 PMID: 10212403
  10. Occurrence and typing of Listeria monocytogenes strains in retail vacuum-packed fish products and in a production plant.
    Int J Food Microbiol. 1999 Mar 1;47(1-2):111-9 PMID: 10357279
  11. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin.
    J Biol Chem. 2001 Sep 14;276(37):34862-70 PMID: 11461915
  12. Adsorption, attachment and biofilm formation among isolates of Listeria monocytogenes using model conditions.
    J Appl Microbiol. 2001 Oct;91(4):725-34 PMID: 11576310
  13. Comparative genomics of Listeria species.
    Science. 2001 Oct 26;294(5543):849-52 PMID: 11679669
  14. Listeria monocytogenes LO28: surface physicochemical properties and ability to form biofilms at different temperatures and growth phases.
    Appl Environ Microbiol. 2002 Feb;68(2):728-37 PMID: 11823213
  15. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds.
    Glycobiology. 2002 Apr;12(4):43R-56R PMID: 12042244
  16. Transfer of microorganisms, including Listeria monocytogenes, from various materials to beef.
    Appl Environ Microbiol. 2002 Aug;68(8):4015-24 PMID: 12147503
  17. Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins.
    Mol Microbiol. 2002 Oct;46(2):587-97 PMID: 12406231
  18. Attachment of Listeria monocytogenes to radish tissue is dependent upon temperature and flagellar motility.
    Appl Environ Microbiol. 2003 Jan;69(1):258-66 PMID: 12514003
  19. Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori.
    Mol Microbiol. 2003 Jun;48(6):1579-92 PMID: 12791140
  20. O-GlcNAc turns twenty: functional implications for post-translational modification of nuclear and cytosolic proteins with a sugar.
    FEBS Lett. 2003 Jul 3;546(1):154-8 PMID: 12829252
  21. Characterization of flagellin expression and its role in Listeria monocytogenes infection and immunity.
    Cell Microbiol. 2004 Mar;6(3):235-42 PMID: 14764107
  22. Structural and genetic characterization of glycosylation of type a flagellin in Pseudomonas aeruginosa.
    J Bacteriol. 2004 May;186(9):2523-31 PMID: 15090491
  23. Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species.
    Nucleic Acids Res. 2004;32(8):2386-95 PMID: 15115801
  24. Role of flagellin and the two-component CheA/CheY system of Listeria monocytogenes in host cell invasion and virulence.
    Infect Immun. 2004 Jun;72(6):3237-44 PMID: 15155625
  25. Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact lymphocytes. Evidence for O-linked GlcNAc.
    J Biol Chem. 1984 Mar 10;259(5):3308-17 PMID: 6421821
  26. Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in humans.
    J Appl Bacteriol. 1987 Jul;63(1):1-11 PMID: 3115937
  27. Cloning and sequence analysis of flaA, a gene encoding a Spirochaeta aurantia flagellar filament surface antigen.
    J Bacteriol. 1989 Mar;171(3):1692-7 PMID: 2921247
  28. Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting.
    J Gen Microbiol. 1988 Aug;134(8):2171-8 PMID: 3150978
  29. Physical and antigenic heterogeneity in the flagellins of Listeria monocytogenes and L. ivanovii.
    J Gen Microbiol. 1988 Sep;134(9):2593-8 PMID: 3151210
  30. Growth of Listeria monocytogenes at refrigeration temperatures.
    J Appl Bacteriol. 1990 Feb;68(2):157-62 PMID: 2108109
  31. Epitope mapping in Salmonella flagellar protein.
    SAAS Bull Biochem Biotechnol. 1991 Jan;4:56-9 PMID: 1370039
  32. Efficiency of sanitizing agents for destroying Listeria monocytogenes on contaminated surfaces.
    J Dairy Sci. 1990 Dec;73(12):3428-32 PMID: 2129169
  33. Cloning and characterization of a gene encoding flagellin of Listeria monocytogenes.
    Mol Microbiol. 1992 Oct;6(20):2919-29 PMID: 1479884
  34. Interaction of Listeria species with human cell monolayers.
    Clin Invest Med. 1994 Feb;17(1):9-17 PMID: 8174317
  35. Pulsed-field fingerprinting of listeriae: identification of genomic divisions for Listeria monocytogenes and their correlation with serovar.
    Appl Environ Microbiol. 1994 Jul;60(7):2584-92 PMID: 8074531
  36. Contamination pattern of Listeria monocytogenes and other Listeria spp. in a salmon slaughterhouse and smoked salmon processing plant.
    Int J Food Microbiol. 1995 Mar;25(1):19-27 PMID: 7599028
  37. Cloning, sequencing, and phenotypic analysis of laf1, encoding the flagellin of the lateral flagella of Azospirillum brasilense Sp7.
    J Bacteriol. 1995 Oct;177(19):5419-26 PMID: 7559324
  38. Use of molecular typing methods to trace the dissemination of Listeria monocytogenes in a shrimp processing plant.
    Appl Environ Microbiol. 1996 Feb;62(2):705-11 PMID: 8593073
  39. Adaptive acid tolerance response in Listeria monocytogenes: isolation of an acid-tolerant mutant which demonstrates increased virulence.
    Appl Environ Microbiol. 1996 May;62(5):1693-8 PMID: 8633868
  40. Identification and characterization of genes required for post-translational modification of Campylobacter coli VC167 flagellin.
    Mol Microbiol. 1996 Jan;19(2):369-78 PMID: 8825781
  41. Analysis of glycoproteins and glycopeptides using fast-atom bombardment.
    Methods Mol Biol. 1996;61:231-41 PMID: 8930877
  42. Biochemical and immunological analyses of the flagellin of Clostridium tyrobutyricum ATCC 25755.
    Microbiol Immunol. 1998;42(1):23-31 PMID: 9525776
  43. Cloning and comparison of fliC genes and identification of glycosylation in the flagellin of Pseudomonas aeruginosa a-type strains.
    J Bacteriol. 1998 Jun;180(12):3209-17 PMID: 9620973
  44. A new class of Caulobacter crescentus flagellar genes.
    J Bacteriol. 1998 Oct;180(19):5010-9 PMID: 9748431
  45. Effect of environmental stress on the ability of Listeria monocytogenes Scott A to attach to food contact surfaces.
    J Food Prot. 1998 Oct;61(10):1293-8 PMID: 9798144
  46. Effect of flagella on initial attachment of Listeria monocytogenes to stainless steel.
    Appl Environ Microbiol. 2000 Feb;66(2):860-3 PMID: 10653766
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.20.6721-6727.2004
Published
2004-10-00
Pages
6721-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC522210
Subset
IM
Databases
GENBANK
AY660548, AY660549, AY660550
External Links
PubMed source DOI: 10.1128/JB.186.20.6721-6727.2004 PMC Full Text View journal details
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