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PMID: 11119490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of flm locus in mesophilic Aeromonas species adherence.

Infection and immunity ·Vol. 69 ·No. 1 ·2001-01-00 ·Pages 65-74

Gryllos I, Shaw JG, Gavín R, Merino S, Tomás JM

Abstract

The adherence mechanism of Aeromonas caviae Sch3N to HEp-2 cells was initially investigated through four mini-Tn5 mutants that showed a 10-fold decrease in adherence. These mutants lost motility, flagella, and their lipopolysaccharide (LPS) O antigen (O-Ag). Three genes, flmB-neuA-flmD, were found to be interrupted by the transposon insertions; additionally, two other genes, one lying upstream (flmA) and one downstream (neuB), were found to be clustered in the same operon. While the flmA and flmB genes were present in all mesophilic Aeromonas spp. (A. hydrophila, A. caviae, A. veronii bv. veronii, and A. veronii bv. sobria) tested, this was not the case for the neuA-flmD-neuB genes. Construction and characterization of flmB insertion mutants in five other mesophilic Aeromonas strains revealed the loss of motility, flagella, and adherence but did not alter the LPS composition of these strains. Taking the above findings into consideration, we conclude (i) that flagella and possibly the LPS O-Ag are involved in the adherence of the mesophilic Aeromonas to human epithelial cells; (ii) flmA and flmB are genes widely distributed in the mesophilic Aeromonas and are involved in flagella assembly, and thus adherence; and (iii) in A. caviae Sch3N the flmA and flmB genes are found in a putative operon together with neuA, flmD, and neuB and are involved in LPS O-Ag biosynthesis and probably have a role in flagellum assembly.

MeSH Terms
Aeromonas/genetics,physiology Bacterial Adhesion Cells, Cultured Centrifugation Chromosome Mapping DNA Transposable Elements Flagellin/analysis,biosynthesis Genes, Bacterial Humans Lipopolysaccharides/analysis Mutation O Antigens/biosynthesis Open Reading Frames
Chemicals
DNA Transposable Elements Lipopolysaccharides O Antigens Flagellin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gryllos I
Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, United Kingdom.
Shaw J G
Gavín R
Merino S
Tomás J M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.69.1.65-74.2001
Published
2001-01-00
Pages
65-74
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97856
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.69.1.65-74.2001 PMC Full Text View journal details
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