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PMID: 15456859 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells.

Molecular and cellular biology ·Vol. 24 ·No. 20 ·2004-10-00 ·Pages 8834-46

Tanaka S, Kamachi Y, Tanouchi A, Hamada H, Jing N, Kondoh H

Abstract

Intermediate-filament Nestin and group B1 SOX transcription factors (SOX1/2/3) are often employed as markers for neural primordium, suggesting their regulatory link. We have identified adjacent and essential SOX and POU factor binding sites in the Nestin neural enhancer. The 30-bp sequence of the enhancer including these sites (Nes30) showed a nervous system-specific and SOX-POU-dependent enhancer activity in multimeric forms in transfection assays and was utilized in assessing the specificity of the synergism; combinations of either group B1 or group C SOX (SOX11) with class III POU proved effective. In embryonic day 13.5 mouse spinal cord, Nestin was expressed in the cells with nuclei in the ventricular and subventricular zones. SOX1/2/3 expression was confined to the nuclei of the ventricular zone; SOX11 localized to the nuclei of both subventricular (high-level expression) and intermediate (low-level expression) zones. Class III POU (Brn2) was expressed at high levels, localizing to the nucleus in the ventricular and subventricular zones; moderate expression was observed in the intermediate zone, distributed in the cytoplasm. These data support the model that synergic interactions between group B1/C SOX and class III POU within the nucleus determine Nestin expression. Evidence also suggests that such interactions are involved in the regulation of neural primordial cells.

MeSH Terms
Animals Base Sequence Binding Sites Cell Nucleus/metabolism DNA-Binding Proteins/genetics,metabolism Embryo, Mammalian/anatomy & histology,physiology Enhancer Elements, Genetic Gene Expression Regulation, Developmental HMGB Proteins High Mobility Group Proteins/genetics,metabolism Humans Intermediate Filament Proteins/genetics,metabolism Mice Molecular Sequence Data Nerve Tissue Proteins/genetics,metabolism Nestin Neurons/cytology,metabolism,physiology Nuclear Proteins/genetics,metabolism POU Domain Factors SOXB1 Transcription Factors SOXC Transcription Factors Sequence Alignment Spinal Cord/cytology,embryology,metabolism Stem Cells/physiology Transcription Factors Transcription, Genetic
Chemicals
DNA-Binding Proteins HMGB Proteins High Mobility Group Proteins Intermediate Filament Proteins NES protein, human Nerve Tissue Proteins Nes protein, mouse Nestin Nuclear Proteins POU Domain Factors SOX2 protein, human SOXB1 Transcription Factors SOXC Transcription Factors Sox11 protein, mouse Sox2 protein, mouse Transcription Factors Pou3f2 protein, mouse
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tanaka Shinya
Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kamachi Yusuke
Tanouchi Aki
Hamada Hiroshi
Jing Naihe
Kondoh Hisato
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.20.8834-8846.2004
Published
2004-10-00
Pages
8834-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC517870
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.24.20.8834-8846.2004 PMC Full Text View journal details
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