Home LiteratureArticle Details
PMID: 1542563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential expression of genes encoding the hypusine-containing translation initiation factor, eIF-5A, in tobacco.

Nucleic acids research ·Vol. 20 ·No. 4 ·1992-02-25 ·Pages 665-9

Chamot D, Kuhlemeier C

Abstract

Two Nicotiana plumbaginifolia cDNA clones, NeIF-5A1 and NeIF-5A2, encoding eukaryotic translation initiation factor eIF-5A (formerly called eIF-4D) were cloned by heterologous screening with Dictyostelium and human eIF-5A probes. eIF-5A is the only protein known to contain a unique amino acid modification, hypusine. Comparison of the Nicotiana deduced amino acid sequences with those of other eIF-5A polypeptides reveals conservation throughout the coding sequence, especially in the region of the hypusine residue. Transcript analysis reveals that NeIF-5A1 is preferentially expressed in photosynthetic tissues, while NeIF-5A2 is constitutively expressed in all plant tissues examined. A polyclonal antibody was raised against NeIF-5A1 overexpressed in E. coli. NeIF-5A1 antiserum crossreacts with an 18 kDa polypeptide doublet in all tobacco tissues examined. At least one polypeptide of ca. 18 kDa from a diversity of higher and lower plants crossreacts with NeIF-5A1 antiserum.

Related Genes
eIF-5A
MeSH Terms
Amino Acid Sequence Blotting, Northern Blotting, Western Cloning, Molecular Eukaryotic Initiation Factor-5 Gene Expression Lysine/analogs & derivatives,genetics Molecular Sequence Data Peptide Initiation Factors/chemistry,genetics Plants, Toxic Sequence Alignment Tobacco/genetics
Chemicals
Eukaryotic Initiation Factor-5 Peptide Initiation Factors hypusine Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chamot D
Institute of Plant Physiology, University of Berne, Switzerland.
Kuhlemeier C
References (22)
22 references, click to expand
  1. Assays for eukaryotic protein synthesis.
    Methods Enzymol. 1979;60:108-23 PMID: 459892
  2. Polyamines in plant physiology.
    Plant Physiol. 1990 Oct;94(2):406-10 PMID: 11537482
  3. The role of mammalian initiation factor eIF-4D and its hypusine modification in translation.
    Biochim Biophys Acta. 1990 Aug 27;1050(1-3):160-2 PMID: 2119810
  4. A small-scale procedure for the rapid isolation of plant RNAs.
    Nucleic Acids Res. 1989 Mar 25;17(6):2362 PMID: 2468132
  5. Protein synthesis initiation factor eIF-4D. Functional comparison of native and unhypusinated forms of the protein.
    J Biol Chem. 1989 Nov 5;264(31):18527-30 PMID: 2509460
  6. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  7. The mammalian hypusine-containing protein, eukaryotic initiation factor 4D. Structural homology of this protein from several species.
    J Biol Chem. 1984 Apr 10;259(7):4563-5 PMID: 6423643
  8. Polyamine binding to proteins in oat and Petunia protoplasts.
    Plant Physiol. 1989;91:738-43 PMID: 11537462
  9. Translational modification of an 18 kilodalton polypeptide by spermidine in rice cell suspension cultures.
    Plant Physiol. 1991 Apr;95(4):1294-7 PMID: 16668128
  10. Divergent genes for translation initiation factor eIF-4A are coordinately expressed in tobacco.
    Nucleic Acids Res. 1991 Oct 25;19(20):5491-6 PMID: 1719476
  11. Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants.
    EMBO J. 1991 Sep;10(9):2635-44 PMID: 1868837
  12. Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Jun;11(6):3105-14 PMID: 1903841
  13. How the messenger got its tail: addition of poly(A) in the nucleus.
    Trends Biochem Sci. 1990 Jul;15(7):277-81 PMID: 1974368
  14. A simple method for the recovery of purified recombinant peptides cleaved from glutathione-S-transferase-fusion proteins.
    Biotechniques. 1991 Feb;10(2):178 PMID: 2059442
  15. The ANB1 locus of Saccharomyces cerevisiae encodes the protein synthesis initiation factor eIF-4D.
    J Biol Chem. 1990 May 25;265(15):8802-7 PMID: 2187871
  16. Sequence determination and cDNA cloning of eukaryotic initiation factor 4D, the hypusine-containing protein.
    J Biol Chem. 1989 Jan 25;264(3):1578-83 PMID: 2492279
  17. The essential role of hypusine in eukaryotic translation initiation factor 4D (eIF-4D). Purification of eIF-4D and its precursors and comparison of their activities.
    J Biol Chem. 1989 Nov 5;264(31):18531-5 PMID: 2509461
  18. cDNA and derived amino acid sequence of the hypusine containing protein from Dictyostelium discoideum.
    FEBS Lett. 1989 Mar 27;246(1-2):94-100 PMID: 2540042
  19. Eukaryotic initiation factor 4D, the hypusine-containing protein, is conserved among eukaryotes.
    J Biol Chem. 1987 Dec 5;262(34):16585-9 PMID: 3119588
  20. The biosynthesis of hypusine (N epsilon-(4-amino-2-hydroxybutyl)lysine). Alignment of the butylamine segment and source of the secondary amino nitrogen.
    J Biol Chem. 1984 Oct 10;259(19):12123-7 PMID: 6434537
  21. Binding of spermidine to a unique protein in thin-layer tobacco tissue culture.
    Plant Physiol. 1988;88:996-8 PMID: 11537444
  22. Isolation and sequence determination of the plant homologue of the eukaryotic initiation factor 4D cDNA from alfalfa, Medicago sativa.
    Plant Mol Biol. 1991 Oct;17(4):927-9 PMID: 1912507
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.4.665
Published
1992-02-25
Pages
665-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312002
Subset
IM
Databases
GENBANK
X63541, X63542, X63543
External Links
PubMed source DOI: 10.1093/nar/20.4.665 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2