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PMID: 15372075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays.

The EMBO journal ·Vol. 23 ·No. 19 ·2004-10-01 ·Pages 3815-24

Angelov D, Verdel A, An W, Bondarenko V, Hans F, Doyen CM, Studitsky VM, Hamiche A, Roeder RG, Bouvet P, Dimitrov S

Abstract

A histone variant H2ABbd was recently identified, but its function is totally unknown. Here we have studied the structural and functional properties of nucleosome and nucleosomal arrays reconstituted with this histone variant. We show that H2ABbd can replace the conventional H2A in the nucleosome, but this replacement results in alterations of the nucleosomal structure. The remodeling complexes SWI/SNF and ACF are unable to mobilize the variant H2ABbd nucleosome. However, SWI/SNF was able to increase restriction enzyme access to the variant nucleosome and assist the transfer of variant H2ABbd-H2B dimer to a tetrameric histone H3-H4 particle. In addition, the p300- and Gal4-VP16-activated transcription appeared to be more efficient for H2ABbd nucleosomal arrays than for conventional H2A arrays. The intriguing mechanisms by which H2ABbd affects both nucleosome remodeling and transcription are discussed.

MeSH Terms
Acetylation Animals Chromatin Assembly and Disassembly/genetics,physiology Chromosomal Proteins, Non-Histone/physiology DNA Footprinting Deoxyribonuclease I Dimerization Histones/metabolism Nuclear Proteins/physiology Nucleosomes/metabolism RNA, Ribosomal, 5S/chemistry Trans-Activators/physiology Transcription Factors/physiology Transcription, Genetic Xenopus/physiology
Chemicals
Chromosomal Proteins, Non-Histone Gal-VP16 Histones Nuclear Proteins Nucleosomes RNA, Ribosomal, 5S SWI-SNF-B chromatin-remodeling complex Trans-Activators Transcription Factors Deoxyribonuclease I
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Angelov Dimitar
Ecole Normale Supérieure de Lyon, CNRS-UMR 5161, Lyon, France.
Verdel André
An Woojin
Bondarenko Vladimir
Hans Fabienne
Doyen Cécile-Marie
Studitsky Vassily M
Hamiche Ali
Roeder Robert G
Bouvet Philippe
Dimitrov Stefan
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600400
Published
2004-10-01
Epub
2004-00-16
Pages
3815-24
Language
English
Region
England
NLM ID
8208664
PMCID
PMC522799
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600400 PMC Full Text View journal details
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