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PMID: 15364901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of mammalian microRNA host genes and transcription units.

Genome research ·Vol. 14 ·No. 10A ·2004-10-00 ·Pages 1902-10

Rodriguez A, Griffiths-Jones S, Ashurst JL, Bradley A

Abstract

To derive a global perspective on the transcription of microRNAs (miRNAs) in mammals, we annotated the genomic position and context of this class of noncoding RNAs (ncRNAs) in the human and mouse genomes. Of the 232 known mammalian miRNAs, we found that 161 overlap with 123 defined transcription units (TUs). We identified miRNAs within introns of 90 protein-coding genes with a broad spectrum of molecular functions, and in both introns and exons of 66 mRNA-like noncoding RNAs (mlncRNAs). In addition, novel families of miRNAs based on host gene identity were identified. The transcription patterns of all miRNA host genes were curated from a variety of sources illustrating spatial, temporal, and physiological regulation of miRNA expression. These findings strongly suggest that miRNAs are transcribed in parallel with their host transcripts, and that the two different transcription classes of miRNAs ('exonic' and 'intronic') identified here may require slightly different mechanisms of biogenesis.

MeSH Terms
Base Sequence DNA Primers Exons Introns MicroRNAs/genetics Molecular Sequence Data Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA Primers MicroRNAs
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rodriguez Antony
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.
Griffiths-Jones Sam
Ashurst Jennifer L
Bradley Allan
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.2722704
Published
2004-10-00
Epub
2004-00-13
Pages
1902-10
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC524413
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.2722704 PMC Full Text View journal details
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