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PMID: 15317798 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription regulation of ezrA and its effect on cell division of Bacillus subtilis.

Journal of bacteriology ·Vol. 186 ·No. 17 ·2004-09-00 ·Pages 5926-32

Chung KM, Hsu HH, Govindan S, Chang BY

Abstract

The EzrA protein of Bacillus subtilis is a negative regulator for FtsZ (Z)-ring formation. It is able to modulate the frequency and position of Z-ring formation during cell division. The loss of this protein results in cells with multiple Z rings located at polar as well as medial sites; it also lowers the critical concentration of FtsZ required for ring formation (P. A. Levin, I. G. Kurster, and A. D. Grossman, Proc. Natl. Acad. Sci. USA 96:9642-9647, 1999). We have studied the regulation of ezrA expression during the growth of B. subtilis and its effects on the intracellular level of EzrA as well as the cell length of B. subtilis. With the aid of promoter probing, primer extension, in vitro transcription, and Western blotting analyses, two overlapping sigmaA-type promoters, P1 and P2, located about 100 bp upstream of the initiation codon of ezrA, have been identified. P1, supposed to be an extended -10 promoter, was responsible for most of the ezrA expression during the growth of B. subtilis. Disruption of this promoter reduced the intracellular level of EzrA very significantly compared with disruption of P2. Moreover, deletion of both promoters completely abolished EzrA in B. subtilis. More importantly, the cell length and percentage of filamentous cells of B. subtilis were significantly increased by disruption of the promoter(s). Thus, EzrA is required for efficient cell division during the growth of B. subtilis, despite serving as a negative regulator for Z-ring formation.

MeSH Terms
Artificial Gene Fusion Bacillus subtilis/cytology,genetics,growth & development Bacterial Proteins/genetics,metabolism Blotting, Western Cell Division/genetics Cytoskeletal Proteins/metabolism Gene Expression Regulation, Bacterial/genetics,physiology Genes, Reporter Mutation Promoter Regions, Genetic Sequence Deletion Transcription Initiation Site Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Proteins Cytoskeletal Proteins EzrA protein, Bacillus subtilis FtsZ protein, Bacteria beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chung Kuei-Min
Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China.
Hsu Hsin-Hsien
Govindan Suresh
Chang Ban-Yang
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.17.5926-5932.2004
Published
2004-09-00
Pages
5926-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC516839
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.186.17.5926-5932.2004 PMC Full Text View journal details
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