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PMID: 12805205 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular architecture of the ATP-dependent CodWX protease having an N-terminal serine active site.

The EMBO journal ·Vol. 22 ·No. 12 ·2003-06-16 ·Pages 2893-902

Kang MS, Kim SR, Kwack P, Lim BK, Ahn SW, Rho YM, Seong IS, Park SC, Eom SH, Cheong GW, Chung CH

Abstract

CodWX in Bacillus subtilis is an ATP-dependent, N-terminal serine protease, consisting of CodW peptidase and CodX ATPase. Here we show that CodWX is an alkaline protease and has a distinct molecular architecture. ATP hydrolysis is required for the formation of the CodWX complex and thus for its proteolytic function. Remarkably, CodX has a 'spool-like' structure that is formed by interaction of the intermediate domains of two hexameric or heptameric rings. In the CodWX complex, CodW consisting of two stacked hexameric rings (WW) binds to either or both ends of a CodX double ring (XX), forming asymmetric (WWXX) or symmetric cylindrical particles (WWXXWW). CodWX can also form an elongated particle, in which an additional CodX double ring is bound to the symmetric particle (WWXXWWXX). In addition, CodWX is capable of degrading EzrA, an inhibitor of FtsZ ring formation, implicating it in the regulation of cell division. Thus, CodWX appears to constitute a new type of protease that is distinct from other ATP-dependent proteases in its structure and proteolytic mechanism.

MeSH Terms
ATP-Dependent Proteases Adenosine Triphosphate/metabolism Bacterial Proteins/genetics,metabolism Binding Sites Heat-Shock Proteins/chemistry,metabolism Macromolecular Substances Protein Structure, Quaternary Serine/chemistry,metabolism Serine Endopeptidases/chemistry,metabolism,ultrastructure
Chemicals
Bacterial Proteins EzrA protein, Bacillus subtilis Heat-Shock Proteins Macromolecular Substances Serine Adenosine Triphosphate ATP-Dependent Proteases CodWX protease Serine Endopeptidases
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Kang Min Suk
NRL of Protein Biochemistry, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
Kim Soon Rae
Kwack Pyeongsu
Lim Byung Kook
Ahn Sung Won
Rho Young Min
Seong Ihn Sik
Park Seong-Chul
Eom Soo Hyun
Cheong Gang-Won
Chung Chin Ha
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg289
Published
2003-06-16
Pages
2893-902
Language
English
Region
England
NLM ID
8208664
PMCID
PMC162141
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg289 PMC Full Text View journal details
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