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PMID: 1530890 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 1 ·1992-01-01 ·Pages 411-5

Whitehouse DB, Putt W, Lovegrove JU, Morrison K, Hollyoake M, Fox MF, Hopkinson DA, Edwards YH

Abstract

A cDNA clone encoding the mRNA for the highly polymorphic human enzyme phosphoglucomutase 1 (PGM1; EC 5.4.2.2) has been isolated and characterized. This was achieved indirectly by first isolating a rabbit cDNA from an expression library using anti-rabbit PGM antibodies. A comparison of the nucleotide sequences shows that the homologies between human and rabbit PGM1 mRNAs are 92% and 97% for the coding nucleotide sequence and the amino acid sequence, respectively. The derived rabbit amino acid sequence is in complete agreement with the published protein sequence for rabbit muscle PGM. A physical localization of the human PGM1 gene to chromosome 1p31 has been determined by in situ hybridization. Analysis of DNA from a wide variety of vertebrates indicates a high level of PGM1 sequence conservation during evolution.

Related Genes
PGM1
MeSH Terms
Animals Base Sequence Blotting, Southern Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 1 Cloning, Molecular DNA/genetics Humans Molecular Sequence Data Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemistry Phosphoglucomutase/genetics Polymerase Chain Reaction RNA, Messenger/genetics Rabbits Sequence Alignment
Chemicals
Oligodeoxyribonucleotides RNA, Messenger DNA Phosphoglucomutase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Whitehouse D B
Medical Research Council Human Biochemical Genetics Unit, Galton Laboratory, University College London, United Kingdom.
Putt W
Lovegrove J U
Morrison K
Hollyoake M
Fox M F
Hopkinson D A
Edwards Y H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.1.411
Published
1992-01-01
Pages
411-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48247
Subset
IM
Databases
GENBANK
M83088
External Links
PubMed source DOI: 10.1073/pnas.89.1.411 PMC Full Text View journal details
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