Immunological detection of human phosphoglucomutase (PGM 1) subtypes.

Forensic science international ·Vol. 41 ·No. 1-2 ·1989-00-00 ·Pages 25-34

Whitehouse DB, Drago GA, Hopkinson DA, Westwood SA, Werrett DJ

Abstract

Anti-phosphoglucomutase (PGM) antibodies have been produced by immunising a sheep with a purified preparation of rabbit skeletal muscle PGM and used to devise an immunological procedure for detecting PGM isozymes after isoelectric focusing. The anti-rabbit PGM antibodies cross react with human PGM and can be used to identify the PGM1 isozymes characteristic of this polymorphism. The patterns revealed by immunodetection are exactly comparable with those obtained by isozyme staining.

MeSH Terms

Animals Blotting, Western Humans Hydrogen-Ion Concentration Isoelectric Focusing Isoenzymes/analysis,genetics Molecular Weight Phosphoglucomutase/analysis,genetics Polymorphism, Genetic Rabbits

Chemicals

Isoenzymes Phosphoglucomutase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Whitehouse D B

MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London, U.K.

Drago G A

Hopkinson D A

Westwood S A

Werrett D J

Article Info

Journal

Forensic science international

Abbr.

Forensic Sci Int

ISSN

0379-0738

DOI

10.1016/0379-0738(89)90232-6

Published

1989-00-00

Pages

25-34

Language

English

Region

Ireland

NLM ID

7902034

Subset

External Links

PubMed source DOI: 10.1016/0379-0738(89)90232-6 View journal details

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Immunological detection of human phosphoglucomutase (PGM 1) subtypes.

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