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PMID: 15304642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 33 ·2004-08-17 ·Pages 12312-7

Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O, Missiakas DM

Abstract

Staphylococcus aureus is the leading cause of wound and hospital-acquired infections worldwide. The emergence of S. aureus strains with resistance to multiple antibiotics requires the identification of bacterial virulence genes and the development of novel therapeutic strategies. Herein, bursa aurealis, a mariner-based transposon, was used for random mutagenesis and for the isolation of 10,325 S. aureus variants with defined insertion sites. By screening for loss-of-function mutants in a Caenorhabditis elegans killing assay, 71 S. aureus virulence genes were identified. Some of these genes are also required for S. aureus abscess formation in a murine infection model.

MeSH Terms
Animals Base Sequence Caenorhabditis elegans/genetics,microbiology DNA, Bacterial/genetics Genes, Bacterial Genes, Regulator Genome, Bacterial Molecular Sequence Data Mutagenesis, Insertional Staphylococcal Infections/etiology Staphylococcus aureus/genetics,growth & development,pathogenicity Virulence/genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bae Taeok
Committee on Microbiology and Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
Banger Alison K
Wallace Adam
Glass Elizabeth M
Aslund Fredrik
Schneewind Olaf
Missiakas Dominique M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0404728101
Published
2004-08-17
Epub
2004-00-10
Pages
12312-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC514475
Subset
IM
Databases
GENBANK
AY672108, AY672109
External Links
PubMed source DOI: 10.1073/pnas.0404728101 PMC Full Text View journal details
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