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PMID: 15289470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The complete genome and proteome of Mycoplasma mobile.

Genome research ·Vol. 14 ·No. 8 ·2004-08-00 ·Pages 1447-61

Jaffe JD, Stange-Thomann N, Smith C, DeCaprio D, Fisher S, Butler J, Calvo S, Elkins T, FitzGerald MG, Hafez N, Kodira CD, Major J, Wang S, Wilkinson J, Nicol R, Nusbaum C, Birren B, Berg HC, Church GM

Abstract

Although often considered "minimal" organisms, mycoplasmas show a wide range of diversity with respect to host environment, phenotypic traits, and pathogenicity. Here we report the complete genomic sequence and proteogenomic map for the piscine mycoplasma Mycoplasma mobile, noted for its robust gliding motility. For the first time, proteomic data are used in the primary annotation of a new genome, providing validation of expression for many of the predicted proteins. Several novel features were discovered including a long repeating unit of DNA of approximately 2435 bp present in five complete copies that are shown to code for nearly identical yet uniquely expressed proteins. M. mobile has among the lowest DNA GC contents (24.9%) and most reduced set of tRNAs of any organism yet reported (28). Numerous instances of tandem duplication as well as lateral gene transfer are evident in the genome. The multiple available complete genome sequences for other motile and immotile mycoplasmas enabled us to use comparative genomic and phylogenetic methods to suggest several candidate genes that might be involved in motility. The results of these analyses leave open the possibility that gliding motility might have arisen independently more than once in the mycoplasma lineage.

MeSH Terms
Amino Acid Sequence Computational Biology Genome, Bacterial Molecular Sequence Data Mycoplasma/genetics Phylogeny Physical Chromosome Mapping Proteome/genetics
Chemicals
Proteome
Authors & Affiliations
19 authors, click to expand affiliations / ORCID
Jaffe Jacob D
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Stange-Thomann Nicole
Smith Cherylyn
DeCaprio David
Fisher Sheila
Butler Jonathan
Calvo Sarah
Elkins Tim
FitzGerald Michael G
Hafez Nabil
Kodira Chinnappa D
Major John
Wang Shunguang
Wilkinson Jane
Nicol Robert
Nusbaum Chad
Birren Bruce
Berg Howard C
Church George M
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.2674004
Published
2004-08-00
Pages
1447-61
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC509254
Subset
IM
Grants
NIAID NIH HHS · R01 AI016478 · United States
NIAID NIH HHS · R37 AI016478 · United States
NIAID NIH HHS · AI16478 · United States
Databases
GENBANK
AE017308
External Links
PubMed source DOI: 10.1101/gr.2674004 PMC Full Text View journal details
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