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PMID: 12466555 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans.

Nucleic acids research ·Vol. 30 ·No. 23 ·2002-12-01 ·Pages 5293-300

Sasaki Y, Ishikawa J, Yamashita A, Oshima K, Kenri T, Furuya K, Yoshino C, Horino A, Shiba T, Sasaki T, Hattori M

Abstract

The complete genomic sequence of an intracellular bacterial pathogen, Mycoplasma penetrans HF-2 strain, was determined. The HF-2 genome consists of a 1 358 633 bp single circular chromosome containing 1038 predicted coding sequences (CDSs), one set of rRNA genes and 30 tRNA genes. Among the 1038 CDSs, 264 predicted proteins are common to the Mycoplasmataceae sequenced thus far and 463 are M.penetrans specific. The genome contains the two-component system but lacks the essential cellular gene, uridine kinase. The relatively large genome of M.penetrans HF-2 among mycoplasma species may be accounted for by both its rich core proteome and the presence of a number of paralog families corresponding to 25.4% of all CDSs. The largest paralog family is the p35 family, which encodes surface lipoproteins including the major antigen, P35. A total of 44 genes for p35 and p35 homologs were identified and 30 of them form one large cluster in the chromosome. The genetic tree of p35 paralogs suggests the occurrence of dynamic chromosomal rearrangement in paralog formation during evolution. Thus, M.penetrans HF-2 may have acquired diverse repertoires of antigenic variation-related genes to allow its persistent infection in humans.

MeSH Terms
Antigenic Variation Antigens, Bacterial/genetics Bacterial Outer Membrane Proteins/genetics,immunology Base Sequence Chromosomes, Bacterial Computational Biology Genome, Bacterial Humans Lipoproteins/genetics,immunology Models, Biological Molecular Sequence Data Mycoplasma penetrans/genetics,immunology,pathogenicity Proteome/genetics Pyrimidines/metabolism Recombination, Genetic Sequence Homology Uridine Kinase/analysis Virulence Factors/genetics
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Lipoproteins P35 protein, bacteria Proteome Pyrimidines Virulence Factors Uridine Kinase
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Sasaki Yuko
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashimurayama, Tokyo, Japan. yuko@nih.go.jp
Ishikawa Jun
Yamashita Atsushi
Oshima Kenshiro
Kenri Tsuyoshi
Furuya Keiko
Yoshino Chie
Horino Atsuko
Shiba Tadayoshi
Sasaki Tsuguo
Hattori Masahira
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkf667
Email
yuko@nih.go.jp
Published
2002-12-01
Pages
5293-300
Language
English
Region
England
NLM ID
0411011
PMCID
PMC137978
Subset
IM
Databases
GENBANK
AP004170, AP004171, AP004172, AP004173, AP004174
External Links
PubMed source DOI: 10.1093/nar/gkf667 PMC Full Text View journal details
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