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PMID: 15282298 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The in vivo activity of Ime1, the key transcriptional activator of meiosis-specific genes in Saccharomyces cerevisiae, is inhibited by the cyclic AMP/protein kinase A signal pathway through the glycogen synthase kinase 3-beta homolog Rim11.

Molecular and cellular biology ·Vol. 24 ·No. 16 ·2004-08-00 ·Pages 6967-79

Rubin-Bejerano I, Sagee S, Friedman O, Pnueli L, Kassir Y

Abstract

Phosphorylation is the main mode by which signals are transmitted to key regulators of developmental pathways. The glycogen synthase kinase 3 family plays pivotal roles in the development and well-being of all eukaryotic organisms. Similarly, the budding yeast homolog Rim11 is essential for the exit of diploid cells from the cell cycle and for entry into the meiotic developmental pathway. In this report we show that in vivo, in cells grown in a medium promoting vegetative growth with acetate as the sole carbon source (SA medium), Rim11 phosphorylates Ime1, the master transcriptional activator required for entry into the meiotic cycle and for the transcription of early meiosis-specific genes. We demonstrate that in the presence of glucose, the kinase activity of Rim11 is inhibited. This inhibition could be due to phosphorylation on Ser-5, Ser-8, and/or Ser-12 because in the rim11S5AS8AS12A mutant, Ime1 is incorrectly phosphorylated in the presence of glucose and cells undergo sporulation. We further show that this nutrient signal is transmitted to Rim11 and consequently to Ime1 by the cyclic AMP/protein kinase A signal transduction pathway. Ime1 is phosphorylated in SA medium on at least two residues, Tyr-359 and Ser-302 and/or Ser-306. Ser-302 and Ser-306 are part of a consensus site for the mammalian homolog of Rim11, glycogen synthase kinase 3-beta. Phosphorylation on Tyr-359 but not Ser-302 or Ser-306 is essential for the transcription of early meiosis-specific genes and sporulation. We show that Tyr-359 is phosphorylated by Rim11.

MeSH Terms
Acetates/metabolism Culture Media/chemistry Cyclic AMP/metabolism Cyclic AMP-Dependent Protein Kinases/metabolism Gene Expression Regulation, Fungal Glucose/metabolism Glycogen Synthase Kinase 3/genetics,metabolism Glycogen Synthase Kinase 3 beta Intracellular Signaling Peptides and Proteins Meiosis/physiology Nitrogen/metabolism Nuclear Proteins/genetics,metabolism Phosphorylation Protein Processing, Post-Translational Protein Serine-Threonine Kinases/genetics,metabolism Saccharomyces cerevisiae/cytology,genetics,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism Second Messenger Systems/physiology Serine/metabolism Transcription Factors/genetics,metabolism Transcription, Genetic Two-Hybrid System Techniques Tyrosine/metabolism
Chemicals
Acetates Culture Media IME1 protein, S cerevisiae Intracellular Signaling Peptides and Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins Transcription Factors Tyrosine Serine Cyclic AMP Glycogen Synthase Kinase 3 beta Protein Serine-Threonine Kinases RIM11 protein, S cerevisiae Cyclic AMP-Dependent Protein Kinases Glycogen Synthase Kinase 3 Glucose Nitrogen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rubin-Bejerano Ifat
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. ykassir@techunix.technion.ac.il
Sagee Shira
Friedman Osnat
Pnueli Lilach
Kassir Yona
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.16.6967-6979.2004
Email
ykassir@techunix.technion.ac.il
Published
2004-08-00
Pages
6967-79
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC479714
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.24.16.6967-6979.2004 PMC Full Text View journal details
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