Abstract
Transcription of the gene encoding sterol regulatory element-binding protein 1c (SREBP-1c) is known to be activated by insulin in the liver. The resultant SREBP-1c protein activates transcription of the genes required for fatty acid synthesis. Here, we use SREBP-1c promoter reporter constructs to dissect the mechanism of insulin activation in freshly isolated rat hepatocytes. The data show that a complete insulin response (increase of 6- to 11-fold) requires two binding sites for liver X receptors (LXRs), which are nuclear receptors that are activated by oxygenated sterols. Disruption of these binding sites did not lower basal transcription but severely reduced the response to insulin. In contrast, disruption of the closely linked binding sites for SREBPs and nuclear factor Y lowered basal transcription drastically but still permitted a 4- to 7-fold increase in response to insulin. Arachidonic acid, an inhibitor of LXR activation, blocked the response to insulin. We conclude that insulin activates the SREBP-1c promoter primarily by increasing the activity of LXRs, possibly through production of a ligand that activates LXRs or their heterodimerizing partner, the retinoid X receptor. Nuclear SREBPs and nuclear factor Y play permissive roles.
MeSH Terms
Animals
Arachidonic Acid/metabolism
CCAAT-Binding Factor/metabolism
CCAAT-Enhancer-Binding Proteins/genetics
Cells, Cultured
DNA-Binding Proteins/genetics
Fatty Acids/biosynthesis
Hepatocytes/cytology,metabolism
Insulin/metabolism,pharmacology
Liver/cytology,physiology
Liver X Receptors
Male
Mice
Orphan Nuclear Receptors
Promoter Regions, Genetic/physiology
RNA, Messenger/analysis
Rats
Rats, Sprague-Dawley
Receptors, Cytoplasmic and Nuclear/metabolism
Receptors, Retinoic Acid/metabolism
Retinoid X Receptors
Sterol Regulatory Element Binding Protein 1
Transcription Factors/metabolism
Transcription, Genetic/drug effects,physiology
Chemicals
CCAAT-Binding Factor
CCAAT-Enhancer-Binding Proteins
DNA-Binding Proteins
Fatty Acids
Insulin
Liver X Receptors
Orphan Nuclear Receptors
RNA, Messenger
Receptors, Cytoplasmic and Nuclear
Receptors, Retinoic Acid
Retinoid X Receptors
Srebf1 protein, mouse
Srebf1 protein, rat
Sterol Regulatory Element Binding Protein 1
Transcription Factors
Arachidonic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chen Guoxun
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390-9046, USA.
Liang Guosheng
Ou Jiafu
Goldstein Joseph L
Brown Michael S
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