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PMID: 15231777 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two genetic loci produce distinct carbohydrate-rich structural components of the Pseudomonas aeruginosa biofilm matrix.

Journal of bacteriology ·Vol. 186 ·No. 14 ·2004-07-00 ·Pages 4457-65

Friedman L, Kolter R

Abstract

Pseudomonas aeruginosa forms biofilms, which are cellular aggregates encased in an extracellular matrix. Molecular genetics studies of three common autoaggregative phenotypes, namely wrinkled colonies, pellicles, and solid-surface-associated biofilms, led to the identification of two loci, pel and psl, that are involved in the production of carbohydrate-rich components of the biofilm matrix. The pel gene cluster is involved in the production of a glucose-rich matrix material in P. aeruginosa strain PA14 (L. Friedman and R. Kolter, Mol. Microbiol. 51:675-690, 2004). Here we investigate the role of the pel gene cluster in P. aeruginosa strain ZK2870 and identify a second genetic locus, termed psl, involved in the production of a mannose-rich matrix material. The 11 predicted protein products of the psl genes are homologous to proteins involved in carbohydrate processing. P. aeruginosa is thus able to produce two distinct carbohydrate-rich matrix materials. Either carbohydrate-rich matrix component appears to be sufficient for mature biofilm formation, and at least one of them is required for mature biofilm formation in P. aeruginosa strains PA14 and ZK2870.

MeSH Terms
Bacterial Proteins/genetics,physiology Biofilms Extracellular Matrix/chemistry,metabolism Gene Deletion Gene Order Genes, Bacterial Mutagenesis, Insertional Mutation Open Reading Frames Polysaccharides, Bacterial/biosynthesis,chemistry,genetics Pseudomonas aeruginosa/cytology,genetics,growth & development,metabolism
Chemicals
Bacterial Proteins Polysaccharides, Bacterial exopolysaccharide, Pseudomonas
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Friedman Lisa
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
Kolter Roberto
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.14.4457-4465.2004
Published
2004-07-00
Pages
4457-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC438632
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058213 · United States
NIGMS NIH HHS · GM58213 · United States
External Links
PubMed source DOI: 10.1128/JB.186.14.4457-4465.2004 PMC Full Text View journal details
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