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PMID: 12810959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Alginate is not a significant component of the extracellular polysaccharide matrix of PA14 and PAO1 Pseudomonas aeruginosa biofilms.

Wozniak DJ, Wyckoff TJ, Starkey M, Keyser R, Azadi P, O'Toole GA, Parsek MR

Abstract

The bacterium Pseudomonas aeruginosa causes chronic respiratory infections in cystic fibrosis (CF) patients. Such infections are extremely difficult to control because the bacteria exhibit a biofilm-mode of growth, rendering P. aeruginosa resistant to antibiotics and phagocytic cells. During the course of infection, P. aeruginosa usually undergoes a phenotypic switch to a mucoid colony, which is characterized by the overproduction of the exopolysaccharide alginate. Alginate overproduction has been implicated in protecting P. aeruginosa from the harsh environment present in the CF lung, as well as facilitating its persistence as a biofilm by providing an extracellular matrix that promotes adherence. Because of its association with biofilms in CF patients, it has been assumed that alginate is also the primary exopolysaccharide expressed in biofilms of environmental nonmucoid P. aeruginosa. In this study, we examined the chemical nature of the biofilm matrix produced by wild-type and isogenic alginate biosynthetic mutants of P. aeruginosa. The results clearly indicate that alginate biosynthetic genes are not expressed and that alginate is not required during the formation of nonmucoid biofilms in two P. aeruginosa strains, PAO1 and PA14, that have traditionally been used to study biofilms. Because nonmucoid P. aeruginosa strains are the predominant environmental phenotype and are also involved in the initial colonization in CF patients, these studies have implications in understanding the early events of the infectious process in the CF airway.

MeSH Terms
Alginates/chemistry,metabolism Carbohydrate Dehydrogenases/metabolism Carbohydrate Metabolism Catechol 2,3-Dioxygenase Cell Division Dioxygenases Drug Resistance, Microbial Extracellular Matrix/chemistry Glucuronic Acid Hexuronic Acids Operon Oxygenases/genetics Plasmids/metabolism Polysaccharides/chemistry Pseudomonas aeruginosa/metabolism Species Specificity Time Factors Uronic Acids/metabolism
Chemicals
Alginates Hexuronic Acids Polysaccharides Uronic Acids Glucuronic Acid Carbohydrate Dehydrogenases GDPmannose dehydrogenase Oxygenases Dioxygenases Catechol 2,3-Dioxygenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wozniak Daniel J
Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208, USA.
Wyckoff Timna J O
Starkey Melissa
Keyser Rebecca
Azadi Parastoo
O'Toole George A
Parsek Matthew R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
2003-06-24
Epub
2003-00-16
Pages
7907-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC164686
Subset
IM
Grants
NIAID NIH HHS · R01 AI051360 · United States
NIAID NIH HHS · 1R01 AIO5 1360-01A1 · United States
NIAID NIH HHS · AI-35177 · United States
NIGMS NIH HHS · 5-T32-GM08449-09 · United States
NHLBI NIH HHS · R01 HL058334 · United States
NHLBI NIH HHS · HL-58334 · United States
NIGMS NIH HHS · T32 GM008449 · United States
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