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PMID: 1518810 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phosphorylation of the retinoblastoma protein by cdk2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 17 ·1992-09-01 ·Pages 7900-4

Akiyama T, Ohuchi T, Sumida S, Matsumoto K, Toyoshima K

Abstract

The retinoblastoma gene product (the RB protein) is phosphorylated in a cell cycle-dependent manner and this modification is believed to be important for cells to progress through the cell cycle. We found that purified cdk2 (cyclin-dependent kinase/cell division kinase 2) can phosphorylate the RB protein in vitro at the sites phosphorylated in the cell. The timing of activation of cdk2 in the cell cycle was similar to that of the onset of phosphorylation of the RB protein. The kinase coprecipitated with the RB protein also exhibited a similar substrate specificity to cdk2 and a similar time course of activation during the cell cycle. We further showed that cdk2 formed a complex with the RB protein in vitro and that its formation was not competitively inhibited by the simian virus 40 large T antigen. These observations suggest that cdk2 or a cdk2-related protein is involved in the cell cycle-dependent phosphorylation of the RB protein.

MeSH Terms
CDC2-CDC28 Kinases Cell Cycle Cells, Cultured Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases Humans In Vitro Techniques Macromolecular Substances Peptide Mapping Phosphorylation Protein Binding Protein Kinases/metabolism Protein Serine-Threonine Kinases Recombinant Proteins/metabolism Retinoblastoma Protein/metabolism
Chemicals
Macromolecular Substances Recombinant Proteins Retinoblastoma Protein Protein Kinases Protein Serine-Threonine Kinases CDC2-CDC28 Kinases CDK2 protein, human Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Akiyama T
Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Japan.
Ohuchi T
Sumida S
Matsumoto K
Toyoshima K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.17.7900
Published
1992-09-01
Pages
7900-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49822
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.17.7900 PMC Full Text View journal details
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