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PMID: 15158473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors.

Biochemical and biophysical research communications ·Vol. 319 ·No. 1 ·2004-06-18 ·Pages 283-8

Zhu J, Xiao G, Xu Y, Yuan F, Zheng C, Liu Y, Yan H, Cole DK, Bell JI, Rao Z, Tien P, Gao GF

Abstract

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GST-fusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.

MeSH Terms
Circular Dichroism Dose-Response Relationship, Drug Escherichia coli/metabolism Glutathione Transferase/metabolism Membrane Glycoproteins/chemistry Peptides/chemistry Protein Conformation Protein Structure, Secondary Protein Structure, Tertiary Recombinant Fusion Proteins/chemistry,metabolism SARS Virus/genetics Spike Glycoprotein, Coronavirus Temperature Viral Core Proteins/chemistry Viral Envelope Proteins/chemistry Viral Fusion Proteins/chemistry Viral Proteins/chemistry
Chemicals
Membrane Glycoproteins Peptides Recombinant Fusion Proteins Spike Glycoprotein, Coronavirus Viral Core Proteins Viral Envelope Proteins Viral Fusion Proteins Viral Proteins spike glycoprotein, SARS-CoV Glutathione Transferase
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Zhu Jieqing
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Xiao Gengfu
Xu Yanhui
Yuan Fang
Zheng Congyi
Liu Yueyong
Yan Huimin
Cole David K
Bell John I
Rao Zihe
Tien Po
Gao George F
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Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
0006-291X
DOI
10.1016/j.bbrc.2004.04.141
Published
2004-06-18
Pages
283-8
Language
English
Region
United States
NLM ID
0372516
PMCID
PMC7111185
Subset
IM
External Links
PubMed source DOI: 10.1016/j.bbrc.2004.04.141 PMC Full Text View journal details
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